2013 ISPAM Mid-Year Mtg Book

Confirmation

Traditional confirmation • Usually involves a combination of biochemical tests such as TSI, LIA, urea in combination with serology (at least poly-O), with additional serology for determining serovars. All equally valid? What’s coming (or is here already) • PCR confirmation from an isolate in lieu of biochemicals and poly-O • Alternate systems for determining serovars – PCR, chemistry platforms

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Putting it all together

• Which steps of the method are most critical? • Time differences?

• Temperature differences? • pH adjustments (or not)? • Do media formulations matter? Are some more inhibitory to Salmonella? • Differences in bias towards or selection for specific serovars? • Other?

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