2013 ISPAM Mid-Year Mtg Book

3/7/2013

 Minimum of 10 artificially inoculated samples  Use CRM, if available  For Qualitative methods inoculate with 1-5 CFU per test portion.  For Quantitative methods, the levels of contamination shall cover the range of the method  Analyse samples on 10 different occasions or days  be performed by at least 2 technicians working independently and with separate samples and reagents .

◦ For Qualitative Methods, artificially inoculate 10 samples at around 10-25 CFU per analytical unit + 5 uninoculated controls ◦ For Quantitative Methods using selective medium, measure repeatability by analyzing 10 replicate samples of a representative food matrix naturally or artificially contaminated

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