2015.14 Vitamin B (July 2018)

2015.14 (JULY 2018) BVit-02 Total B_AOAC_2015.14_MLT_Final

FOR ERP USE ONLY DO NOT DISTRIBUTE

11.Multi-position magnetic stir plate. 12.Room Light Shields: A.L.P. Protect-A-Lamp, UV cutoff at 460 nm, or equivalent. 13.Graduated cylinders: various sizes, including: 10, 100, 500, and 1000 mL. 14.Beakers: various sizes, including: 100, 200, 400, 600, 1000, and 2000 mL. 15.Volumetric flasks: various sizes, including: 10, 25, 50, 100, 250, and 2000 mL. 16.Mobile phase bottles: glass, various sizes, including: 250, 500, 1000, and 2000 mL . 17.Disposable, plastic Pasteur pipettes. 18.Amber bottles: volume capacity of 50 and 100 mL (for stock standard storage). 19.Weighing vessels: various, including disposable weighing boats and glass weighing funnels. 20.Positive displacement pipettes: Gilson Microman - 10, 100, 250, and 1000 µL; Part #’s F148501, F148504, F148505, and F148506. 21.Positive displacement pipette tips: Gilson Capillary Piston - 10, 100, 250, and 1000 µL; Part #’s F148312, F148314, F148014, and F148560. 22.Plastic syringes, 3 mL. 23.Syringe filters, PTFE 0.45 µm syringe filters: Acrodisc 25 mm, or equivalent. 24.50 mL self-standing, plastic centrifuge tubes: Superior Scientific, Ltd., or equivalent. 25.Autosampler vials: Waters autosampler vials; 9 mm amber with screw top 12x32 mm pre-split PTFE- silicon septa; Waters Part # 186000847C, or equivalent. 26.Teflon coated magnetic stir bars. C. Reagents 1. Nicotinamide: USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot; Catalog # 1462006. Store as indicated on label. 2. Niacin (nicotinic acid): USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot; Catalog # 1461003. Stored as indicated on label. 3. Pyridoxine hydrochloride: USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot; Catalog # 1587001. Store in desiccator protected fromwhite light. Dry according to manufacturer’s instructions prior to use. 4. Riboflavin: USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot; Catalog # 1603006. Store in desiccator protected from white light. Dry according to manufacturer’s instructions prior to use. 5. Thiamine hydrochloride: USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot; Catalog # 1656002. Store in desiccator protected fromwhite light. Measure the moisture content of the powder prior to use. 6. Pyridoxamine dihydrochloride: Fluka Analytical Standard, catalog# P9380. 7. Pyridoxal hydrochloride: Sigma, catalog# P9130. 8. 2 H 4 -Nicotinamide: CDN Isotopes; Catalog # D-3457. 9. 2 H 4 -Nicotinic acid: CDN Isotopes; Catalog # D-4368.