2015.14 Vitamin B (July 2018)

2015.14 (JULY 2018) BVit-02 Total B_AOAC_2015.14_MLT_Final

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limits established by a control chart. By and large, the levels reported by this method are within the NIST certified range because of the minute concentration of phosphorylated vitamin forms in SRM 1849a.

F.

Calculations 1. Vitamin Stock Solutions concentration calculation [ ] Vol s Stk =

PSMW Vit 000 ,1 ××× ×

Where, [Vit] Stk :

Vitamin standard stock solution concentration, in μg/mL.

:

Weight of standard in mg.

W S

M:

Moisture content correction factor for the standard, if applicable.

S:

Stoichiometric correction factor, if applicable.

P:

Purity of standard as defined by the manufacturer.

1,000:

Units conversion factor, from mg to µg.

Vol:

Dissolution volume in mL.

2. Calculation of vitamin concentrations in the Mixed Working Standard (MWS)

Vol

MWS ] [

Stk ] [ ×

Vit

Vit

=

10

mL

Where, [Vit] MWS :

Vitamin concentration in the Mixed Working Standard in ng/mL.

:

Concentration of Vitamin Stock Standard in μg/mL

[Vit] Stk

Vol:

Volume of stock solution added to MWS in μL.

3. Calculation of Working Standard concentration

] [ MWS

Vol

Vit

×

] [ WSx

Vit

=

MWS

500

Where,