2015.14 Vitamin B (July 2018)

2015.14 (JULY 2018) BVit-02 AOAC 2015.014 B3 Accuracy FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 1

Accuracy ‐ Spike Recovery

Spike Recovery

Product

SPIFAN Code

Analyte

Nr. replicates (n)

Native level ug/100g as RTF

Low (~50% Fort.)

High (~200% Fort.)

Avg (%)

RSD % Avg (%) RSD (%)

MEAN

98.1

2.1 7.8 5.9 6.3 6.8 1.8 2.1 1.6 5.8 4.8

97.8

2.3 6.8 3.8 5.3 4.9 2.4 2.4 3.3 7.0 4.5

Adult Nutritional RTF High Fat, placebo Adult Nutritional RTF High Protein, placebo

00820RF00 00821RF00 00847RF00 410057652Z 410457651Z 00796RF

Total B3 Total B3 Total B3 Total B3 Total B3 Total B3 Total B3 Total B3

3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps

34.2 24.4 10.4

102.1 101.7 105.4 104.5 103.0 102.3 101.8 105.3 103.2

103.1 102.9 105.7 103.8 110.7 105.6 100.3 103.4 104.4

Child Formula Powder, placebo Infant Elemantal Powder, placebo

5.7

Infant Formula Powder Partially Hydrolyzed Milk Based Infant Formula Powder Partially Hydrolyzed Soy Based

775.0 787.0 843.0

Infant Formula Powder Soy Based Infant Formula RTF Milk Based, placebo

E10NWZC EV4H2Q

23.2

MEAN

Additional information to be completed by SD

Were all spike recovery samples prepared on multiple days? Were spike recoveries calculated from theoretical additions? Or was spiking solution analyzed and the results used to calculate spike recoveries? What form of the nutrient was spiked into the product? What kind of solution or solvent was used to deliver the spike to the product? Was a portion of the product spiked? Were all spike recovery samples taken from the same spiked product? Were multiple portions of the product spiked and each spiked product analyzed? Were products weighed into extraction vessels before spiking? Were products spiked before they were weighed into extraction vessels? Were all spike recovery samples prepared on the same day?

yes no yes yes

no no

yes yes no

Mixed std

water

What percentage of the spiked sample was the spike blanc? (How much was product diluted by spike?)

3% low‐13% high