2017.03 Amino Acids (Tryptophan) - Amino-02

2017.03 (JULY 2018) Amino-02 TRP_AOAC_2017.03_MLT_Corrected 080218

FOR ERP USE ONLY DO NOT DISTRIBUTE

2. Precision:

Table 3

Precision - repeatability.

Product

SPIFAN Code Analyte

Nr. replicates (n) Conc. Level

RSDr

RSD iR

Conc. Level RSDr

RSD iR

REQUIREMENTS SMPR

SRM1849a

CLC10-B Tryptophan

12 12 12 12 12 12 12 12 12 12 12 12 12 12

20.1 25.3 18.3 25.7 24.1 21.4 23.7 32.2 23.2 19.5 18.3 22.4 70.6 91.3

0.4 0.6 5.0 0.8 0.2 0.9 0.9 0.2 2.0 0.3 0.2 0.5 1.9 0.3

1.5

X

≤Z % ≤W%

Infant FormulaPowderPartially Hydrolyzed Milk Based 410057652Z Tryptophan

1.0 Analyte 1 6.9 Analyte 2 2.0 Analyte 3 1.8 Analyte 4 1.7 Analyte 5 1.3 Analyte 1 1.6 Analyte 2 2.4 Analyte 3 1.7 Analyte 4 2.3 Analyte 5 1.8 Analyte 1 3.7 Analyte 2 1.9 Analyte 3 Analyte 4 Analyte 5 Analyte 1 Analyte 2 Analyte 3 Analyte 4 Analyte 5

0.4-5.0

4%

Infant FormulaPowderPartially Hydrolyzed Soy Based

410457651Z Tryptophan 4052755861 Tryptophan 4044755861 Tryptophan 00859RF00 Tryptophan 00866RF00 Tryptophan 00795RF Tryptophan 50350017W1 Tryptophan K16NTAV Tryptophan E10NWZC Tryptophan EV4H2R Tryptophan 00730RF00 Tryptophan 00729RF00 Tryptophan

ToddlerFormulaPowderMilk-Based Infant FormulaPowderMilk-Based Adult Nutritional PowderLow Fat

Child FormulaPowder Infant Elemental Powder

5.0-50

3%

Infant FormulaPowderFOS/GOS Based Infant FormulaPowderMilk Based Infant FormulaPowderSoy Based Infant FormulaRTF Milk Based Adult Nutritional RTF High Prtotein Adult Nutritional RTF High Fat

50-2500

2%

Global Mean %

1.0

2.3

H. SPECIFICITY - Selectivity for the analyte of interest is supported by the following: 1. The selectivity of chromatographic separation combined with native fluorescence 2. Near 100% recovery of reference material