2017.03 Amino Acids (Tryptophan) - Amino-02

2017.03 (JULY 2018) Amino-02 Tryptophan by enz hyd_Abbott Labs_Method

FOR ERP USE ONLY DO NOT DISTRIBUTE HPLC Determination of Total Tryptophan in Infant Formula and Adult/Pediatric Nutritional Formula Following Enzymatic Hydrolysis Abbott Nutrition, Division of Abbott Laboratories

GH . METHOD PERFORMANCE (Data from AMINO-02 AOAC SLV) 1. Accuracy:

Table 1

Accuracy - Spike Recovery

Spike Recovery

Product

SPIFAN Code

Analyte

Nr. replicates (n)

+ Spike Level 1

+ Spike Level 2

Native level

Avg (%) RSD % Avg (%) RSD (%)

SRM1849a

CLC10-B

TRP TRP TRP TRP TRP TRP TRP TRP

12 12 11 12 10 12 12 12

20.1 25.3 18.3 21.4 32.2 19.5 18.3 91.3

99.9

1.8 3.3 8.6 5.2 2.5 3.0 5.1 1.6

100.9 104.1 101.5

2.4 2.7 4.8 3.9 2.7 2.0 3.5 1.3

Infant Formula Powder Partially Hydrolyzed Milk Based Infant Formula Powder Partially Hydrolyzed Soy Based

410057652Z 410457651Z 00859RF00 00795RF K16NTAV E10NWZC 00729RF00

104.3 103.7

Adult Nutritional Powder Low Fat

96.2

99.5

Infant Elemental Powder

100.7 100.5

102.4 101.7

Infant Formula Powder Milk Based Infant Formula Powder Soy Based Adult Nutritional RTF High Fat

93.8

99.5

104.8

104.9

Global Mean % 100.5

3.9 101.8

2.9

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