2017.03 Amino Acids (Tryptophan) - Amino-02

2017.03 (JULY 2018) Amino-02 TRP_AOAC_2017.03_MLT_Corrected 080218

FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 4: Statistical Analysis of MLT Blind Duplicates Sample ID: K16NTAV

E10NWZC

410057652Z

410457651Z

00729RF00

Total number of laboratories Total number of replicates

10 20

10 20

10 20

10 20

9

18

Overall mean of all data (grand mean), mg/100g Repeatability standard deviation Reproducibility standard deviation Repeatability relative standard deviation, % Reproducibility relative standard deviation, %

19.56

18.09

25.41

17.92

90.52

0.50 0.79

0.33 0.73

0.23 1.08

0.65 1.78

1.22 3.72

2.5 4.1

1.8 4.0

0.9 4.2

3.6 9.9

1.3 4.1

HORRAT value

0.56

0.55

0.61

1.35

0.72

Sample ID:

00730RF00

4052755861

4044755861

EV4H2R

CLC10-B

Total number of laboratories Total number of replicates

8

10 20

10 20

10 20

10 20

16

Overall mean of all data (grand mean), mg/100g Repeatability standard deviation Reproducibility standard deviation Repeatability relative standard deviation, % Reproducibility relative standard deviation, %

70.42

24.79

23.96

21.99

18.14

1.45 2.97

0.44 1.00

0.51 0.79

0.22 0.88

0.29 0.55

2.1 4.2

1.8 4.0

2.1 3.3

1.0 4.0

1.6 3.0

HORRAT value

0.71

0.58

0.47

0.56

0.41

Sample ID:

00866RF00

00859RF00

00795RF00

50350017W1

Total number of laboratories Total number of replicates

10 20

9

10 20

10 20

18

Overall mean of all data (grand mean), mg/100g Repeatability standard deviation Reproducibility standard deviation Repeatability relative standard deviation, % Reproducibility relative standard deviation, %

23.38

21.35

32.80

22.59

0.60 1.02

0.32 0.67

0.58 1.09

0.50 0.80

2.5 4.4

1.5 3.1

1.8 3.3

2.2 3.5

HORRAT value

0.62

0.44

0.50

0.50

Repeatability (RSD r )

For all fourteen of the product matrices analyzed, the overall repeatability averaged 2.1% RSD with a range of 0.9 – 3.6% RSD. The SMPR requirements of ≤ 3% RSD for samples with L-tryptophan levels of 5-50 mg/100g RTF or ≤ 2% RSD for products with levels ranging from 50 - 2500 mg/100g RTF were met for thirteen of the fourteen matrices analyzed. All SPIFAN II product matrices except 00730RF00 and 00729RF00 had L- tryptophan levels between 5-50 mg/100g RTF. The L-tryptophan levels in 00730RF00 and 00729RF00 are in