Contemporary Resume
Michael Patrick Valley
Home
Work
5463 Shale Road
2800 Woods Hollow Road
Fitchburg, WI 53711
Madison, WI 53711
(608) 332-3397
(608) 274-1181 ext 1533 mike.valley@promega.com
valmibeaur@yahoo.com
Current Position
Senior Scientist I in the Assay Design Group of R&D at Promega
Technical Skills
Design of various luminescent assays
Mammalian cell culture, including various 3D cell culture methods Site-directed mutagenesis and subcloning of genes for overexpression in Escherichia coli Protein purification including anion exchange, hydrophobic, affinity, and size exclusion chromatographies Kinetics (steady state, stopped-flow, and rapid quench) including anaerobic work, isotope effects, and pH and temperature dependencies Enzyme inhibition and substrate rescue studies Directed evolution of enzymatic activity including genetic knockouts in Escherichia coli UV/visible, fluorescence, resonance Raman, 1D NMR, and EPR spectroscopies Synthesis and purification of substrates Senior Research Scientist (November 2004 – present) Research and Development, Promega, Madison, Wisconsin – design of luminescent assays to measure enzyme activities, transcriptional reporters, and cellular viability . Postdoctoral Research Associate (June 2001 – October 2004) Dr. Paul F. Fitzpatrick, Biochemistry and Biophysics, Texas A&M University, College Station, Texas - chemical, kinetic, and structural characterization of the flavoenzyme nitroalkane oxidase . Dr. John D. Lipscomb, Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota - spectroscopy, mutagenesis and structure-function studies of the metalloenzyme protocatechuate 3,4-dioxygenase. Industrial Internship (August – November 1998) Dr. F. Sima Sariaslani, E.I. DuPont Central Research & Development, Wilmington, Delaware - protein purification and characterization. Research Experience Graduate Student (August 1994 – May 2001)
Education
University of Minnesota, Minneapolis, Minnesota, Ph.D. in Biochemistry, Molecular Biology and Biophysics, July 2002, GPA 4.0/4.0.
University of Illinois, Champaign, Illinois, B.S. in Chemistry, May 1994, GPA 5.0/5.0, summa cum laude with highest distinction.
San Jose State University, San Jose, California, Department of Energy sponsored six week course in Nuclear and Radiochemistry, Summer 1992.
Made with FlippingBook flipbook maker