Contemporary Resume

Michael Patrick Valley

Home

Work

5463 Shale Road

2800 Woods Hollow Road

Fitchburg, WI 53711

Madison, WI 53711

(608) 332-3397

(608) 274-1181 ext 1533 mike.valley@promega.com

valmibeaur@yahoo.com

Current Position

Senior Scientist I in the Assay Design Group of R&D at Promega

Technical Skills



Design of various luminescent assays

 Mammalian cell culture, including various 3D cell culture methods  Site-directed mutagenesis and subcloning of genes for overexpression in Escherichia coli  Protein purification including anion exchange, hydrophobic, affinity, and size exclusion chromatographies  Kinetics (steady state, stopped-flow, and rapid quench) including anaerobic work, isotope effects, and pH and temperature dependencies  Enzyme inhibition and substrate rescue studies  Directed evolution of enzymatic activity including genetic knockouts in Escherichia coli  UV/visible, fluorescence, resonance Raman, 1D NMR, and EPR spectroscopies  Synthesis and purification of substrates  Senior Research Scientist (November 2004 – present) Research and Development, Promega, Madison, Wisconsin – design of luminescent assays to measure enzyme activities, transcriptional reporters, and cellular viability .  Postdoctoral Research Associate (June 2001 – October 2004) Dr. Paul F. Fitzpatrick, Biochemistry and Biophysics, Texas A&M University, College Station, Texas - chemical, kinetic, and structural characterization of the flavoenzyme nitroalkane oxidase . Dr. John D. Lipscomb, Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota - spectroscopy, mutagenesis and structure-function studies of the metalloenzyme protocatechuate 3,4-dioxygenase.  Industrial Internship (August – November 1998) Dr. F. Sima Sariaslani, E.I. DuPont Central Research & Development, Wilmington, Delaware - protein purification and characterization. Research Experience  Graduate Student (August 1994 – May 2001)

Education

 University of Minnesota, Minneapolis, Minnesota, Ph.D. in Biochemistry, Molecular Biology and Biophysics, July 2002, GPA 4.0/4.0.

 University of Illinois, Champaign, Illinois, B.S. in Chemistry, May 1994, GPA 5.0/5.0, summa cum laude with highest distinction.

 San Jose State University, San Jose, California, Department of Energy sponsored six week course in Nuclear and Radiochemistry, Summer 1992.