Contemporary Resume

 Fitzpatrick, P.F., Orville, A.M., Nagpal, A., and Valley, M.P. “Nitroalkane oxidase, a carbanion-forming flavoprotein homologous to acyl-CoA dehydrogenase.” Arch.Biochem.Biophys. (2005) 433: 157-65.

 Nagpal, A., Valley, M.P., Fitzpatrick, P.F., and Orville, A.M. “Crystallization and preliminary analysis of active nitroalkane oxidase in three crystal forms.” Acta.Crystallogr.D.Biol.Crystallogr. (2004) 60: 1456-60.

 Valley, M.P. and Fitzpatrick, P.F. “Comparison of Enzymatic and Non-Enzymatic Nitroethane Anion Formation: Thermodynamics and Contribution of Tunneling.” J.Am.Chem.Soc. (2004) 126 :6244-6245 .

 Valley, M.P. and Fitzpatrick, P.F. “Reductive Half-Reaction of Nitroalkane Oxidase: Effect of Mutation of the Active Site Aspartate to Glutamate.” Biochemistry (2003) 42 :5850-5856.

 Valley, M.P. and Fitzpatrick, P.F. “Inactivation of Nitroalkane Oxidase upon Mutation of the Active Site Base and Rescue with a Deprotonated Substrate.” J.Am.Chem.Soc. (2003) 125 :8738-8739.

 Daubner, S.C., Gadda, G., Valley, M.P., and Fitzpatrick, P.F. “Cloning of Nitroalkane Oxidase from Fusarium oxysporum Identifies a New Member of the Acyl-CoA Dehydrogenase Superfamily.” Proc.Natl.Acad.Sci.U.S.A. (2002) 99 :2702-2707.  Valley, M.P., Burk, D., Frazee, R.W., Yu, H., Dolbeare, K.B., Ohlendorf, D.H., and Lipscomb, J.D. “Differences in Single versus Multiple Turnover of a Mutant Protocatechuate 3,4-Dioxygenase: A Short Circuit in Catalysis.” In preparation.  Vetting, M.W., Valley, M.P., D’Argenio, D., Ornston, L.N., Lipscomb, J.D., and Ohlendorf, D.H. “Biophysical Analysis of R457S and R133H Mutants of Protocatechuate 3,4-Dioxygenase from Acinetobacter Strain ADP1.” In preparation.  Valley, M.P., Sobol, M., Karassina, N., Duellman, S., Leippe, D., and Vidugiriene, J. “New Bioluminescent Assays Enable Easy Measurement of Glucose-Dependent Metabolic Pathways” Fifth Annual SLAS Conference, San Diego, CA. January 23-27, 2016.  Valley, M.P., Sobol, M., Karassina, N., Duellman, S., Leippe, D., Aoyoma, N., Hancock, M., Carlson, C., and Vidugiriene, J. “Sensitive and Easy to Use Bioluminescent Assays for Measuring Glucose Dependent Metabolic Pathways” 75 th Scientific Sessions, Boston, MA. June 5-9, 2015.  Valley, M.P., Kupcho, K.R., Zimprich, C.A., Leippe, D., Niles, A.L., Vidugiriene, J., Cali, J.J., Kelm, J.M., Moritz, W., and Lazar, D.F. “Luminescent Cell Health Assays for Tumor Spheroid Evaluation” AACR Annual Meeting 2014, San Diego, CA. April 5-9, 2014.  Cali, J.J., Valley, M.P., Unch, J., Meisenheimer, P.L., and Lazar, D.F. “A Novel Cell Based ATP Assay with Enhanced Operational Stability and Ease of Use” Third Annual SLAS Conference, San Diego, CA. January 18- 22, 2014.  Riss, T.L., Valley, M.P., Zimprich, C.A., Niles, A.L., Kupcho, K.R., and Lazar, D.F. “Design and Validation of Bioluminescent Assays for 3D Cell Culture Models” Third Annual SLAS Conference, San Diego, CA. January 18-22, 2014. Abstracts  Valley, M.P., Sobol, M., Karassina, N., Duellman, S., Cali, J.J., and Vidugiriene, J. “A Bioluminescent Assay for Measuring Glucose Uptake” AACR Annual Meeting 2015, Philadelphia, PA. April 18-22, 2015.

 Valley, M.P., Zimprich, C., Cali, J.J., and Lazar, D.F. “A Bioluminescent Cell Viability Assay Optimized for 3D Microtissues” AACR Annual Meeting 2013, Washington D.C. April 6-10, 2013.