2018 Sugar ERP - Method Review Book

AOAC SMPR ERPs - 2018 METHOD REVIEW FORM

Submission ID

4096451708597282986

Submission Date

2018-08-09 12:32:50

Name

Nicole Burke

E-mail

nicole.burke@kellogg.com

Organization

Kellogg

Title of Method

Analysis of the Labeling Sugars in Feed, Food, and Ingredients

AOAC Candidate Method Number (e.g. ALN-01)

SUG-001

Applicable SMPR

2018.001 Standard Method Performance Requirements for Sugars in Animal Feed, Pet Food, and Human Food

I. Summary of the Method

This method utilizes normal phase high performance liquid chromatography with refractive index detection to determine the quantities of six sugar analytes used in nutritional labeling: fructose, glucose, galactose, sucrose, maltose, and lactose. The method is capable of quantifying these sugars at levels above 0.1% in animal feed, pet food, and human food. Sugar is extracted from these sample matrices using water as an extraction solvent. The resulting extracts are diluted with acetonitrile, filtered with a 0.2 µm membrane filter, and then separated on an amino column prior to detection by refractive index. Quantitation is calculated on the basis of sample response relative to a ribose internal standard and linear regressions for standards of each sugar analyte. The applicability of the method does not fully support the applicability of the SMPR. The SMPR states that the method must be able to individually measure fructose, galactose, glucose, sucrose, maltose, and lactose; however, the method does not fully demonstrate the ability to individually measure galactose up to the standards of the SMPR. Additionally, while the SMPR states that the method must be able to perform these analyses in selected ingredients and foods consumed by animals, pets, and humans, the method in question only demonstrates analysis in a pet food matrix for sucrose and not all of the sugars specified in the SMPR. Lastly, the SMPR states that the method must account for a variety of potential inferences. While the method provides a table of retention times for a variety of potential inferences, it does not provide any further evidence demonstrating that the method can account for interferences in quantitation. Additionally, the method only provides this retention time information for a small group of analytes that does not represent all, or even a majority, of the potential interferants specified in Table 2. of the SMPR. Yes, the technique of Refractive Index-High Performance Liquid Chromatography utilizing normal phase chromatography could meet the SMPR if it is able to meet the method performance requirements specified in the SMPR for the six analytes. The ability of the method to meet these performance requirements is discussed below in Section III.

II. Review of the Method Only 1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing.

2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR.