2018 Sugar ERP - Method Review Book

4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s). III. Review of Supporting Information 1. Are the definitions specified in the SMPR used and applied appropriately in the supporting documentation (manuscripts, method studies, etc...)? If not, please explain the differences and if the method is impacted by the difference. 2. Is there information demonstrating that the method meets the SMPR Method Performance Requirements using the Reference Materials stated in the SMPR? If not, then specify what is missing and how this impacts demonstration of performance of the method. method performs within the SMPR Method Performance REquirements table specifications for all analytes in the SMPR applicability statement? If not, please specify what is missing and whether or not the method's applicability should be modified. 3. Is there information demonstrating that the

Yes.

All definitions are used correctly except for reproducibility. The reported RSDR study was performed on 4 separate occasions by 2 independent analysts. While these results should remain in the report as an intermediate precision study, a broader number of variables (different locations, equipment, reagents, and analysts) needs to be performed to fully estimate method reproducibility (RSDR).

A Certified Reference Material was listed as not being currently available.

The method measured total fructans as total fructans including inulin, levan, branched fructans, agavins (agave fructans), and FOS as listed in SMPR.