2018 Sugar ERP - Method Review Book

AOAC SMPR ERPs - 2018 METHOD REVIEW FORM

Submission ID

4101689242421387495

Submission Date

2018-08-15 14:02:04

Name

Hari Narayanan

E-mail

hari@metrohmusa.com

Organization

Metrohm USA Inc

Title of Method

Sugar Profile by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection

AOAC Candidate Method Number (e.g. ALN-01)

SUG-003

Applicable SMPR

AOAC SMPR® 2018.001

I. Summary of the Method

Method tested and validated for wide range of food matrices covering the AOAC food pyramid. Single chromatographic condition and optimized sample preparation based on protein/sugar content is applied to 17 types of sample matrices and the multi lab results are presented in this method. Pulse ampherometric detection is highly selective and sensitive and it is being widely used in food and pharmaceutical industry for wide range of sugar analysis.

Efforts and time taken to develop and perform multi lab validation is commendable and the method author is highly appreciated for the efforts.

II. Review of the Method Only 1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR.

Method fulfills the SMPR requirements for majority of samples. Few Areas of concern explained in other sections include, safety, specificity, accuracy and precision for some sugar analytes in few sample matrices. Couple of references 3,4 mentioned in Section E of this method. But no detailed reference is available within submission.

Anion exchange chromatography together with Pulse ampherometric detection offers selectivity and sensitivity that can meet SMPR's requirements.

This particular method - tries to apply a single gradient method to various food matrices. This can lead to specificity loss in many cases, depending on the presence of the interference listed in SMPR - Figure 2. For example - obvious critical pair is observed between Arabinose, the internal standard used, and Glocosamine interference listed in SMPR. Also the resolution of Lactose is not sufficient in presence of lactulose and isomaltose. Probably this could a reason why Lactose and Sucrose are outliers for CRM BCR-644.

Baseline drift due to gradient is visible in the Lactose & Maltose RT. A visible shoulder peak also noticed very close to Lactose in several sample matrices.