2019 Vet Drug Residues ERP - Review Book

AOAC SMPR ERPs - 2019 METHOD REVIEW FORM

Submission Date

2019-09-09 02:30:09

Name

JOE BOISON

E-mail

jboison@shaw.ca

Organization

Independent Consultant

Title of Method

VETERINARY DRUGS IN FOODS BY LC-MS/MS

AOAC Candidate Method Number (e.g. ALN-01)

VDR-001

Applicable SMPR

VDR-001

I. Summary of the Method

The method uses 4 methods to screen for and confirm a complement of 154 veterinary drug residue in milk-based products (skimmed milk powder, fat-filled milk powder, whey protein, lactose, casein, infant formula, infant cereals and baby foods), meat-based and fish-based products (fresh, powdered, cooked, infant cereals and baby foods by LC-MS/MS. The method involves the extraction of the food matrix with a mixture of water, acetonitrile and formic acid followed by a liquid-liquid partitioning with a mixture of sodium sulfate, sodium chloride and citrate salts. After centrifugation, the resulting acetonitrile supernatant is diverted to 4 portions. The first portion can be split into 2 to permit the analysis of 99 multi-analyte drug residue (1 portion) and 6 avermectins (2nd portion). YE S. 105 VETERINARY DRUGS – [SHOULD BE REVISED TO ACCOUNT FOR MONITORING THE APPROPRIATE MARKER RESIDUES] - milk based products including milk fractions, intact formula, growing up formula, adult formula, infant cereals, and baby foods - meat and fish-based products including powdered fresh, cooked, infant cereals and baby foods 23 BETA LACTAMS – - for powdered samples (infant formula, milk fractions, infant cereals, meat and fish powders…….) - for fresh meat/seafood/fish products; - baby food jars (eg., puree) - 12 (14) AMINOGLYCOSIDES – Sample preparation described for - milk-based products – milk fractions, infant formulae, infant cereals, baby foods - meat-based, fish-based, seafood-based products including powdered, fresh, , infant cereals and baby foods cooked meat/fish/seafood - egg-based products including egg white and whole egg powders [GENTAMYCIN SHOULD BE COUNTED AS 1 EVEN THOUGH THE 3 ISOMERS ARE BEING MEASURED BECAUSE THEIR PRESENCE NEED TO BE SUMMED UP] 5 TETRACYCLINE RESIDUES & THEIR EPIMERS – - Applicable matrices not spelled out as the previous methods. [SHOULD BE INCLUDED IN THE SOP]

II. Review of the Method Only 1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing.