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Table 2013.11B. Summary of results for the detection of Listeria monocytogenes in queso fresco (125 g) Method a VIDAS LMX w/OXA VIDAS LMX w/ALOA Inoculation level Uninoculated Low High Uninoculated Low High Candidate presumptive positive/total No. samples analyzed 0/144 70/144 144/144 0/144 70/144 144/144 Candidate presumptive POD (CP) 0.00 0.49 1.00 0.00 0.49 1.00 (0.00, 0.03) (0.40, 0.57) (0.97, 1.00) (0.00, 0.03) (0.40, 0.57) (0.97, 1.00) s r b 0.00 0.51 0.00 0.00 0.51 0.00 (0.00, 0.16) (0.46, 0.52) (0.00, 0.16) (0.00, 0.16) (0.46, 0.52) (0.00, 0.16) s L c 0.00 0.00 0.00 0.00 0.00 0.00 (0.00, 0.16) (0.00, 0.12) (0.00, 0.16) (0.00, 0.16) (0.00, 0.12) (0.00, 0.16) s R d 0.00 0.51 0.00 0.00 0.51 0.00 (0.00, 0.22) (0.46, 0.52) (0.00, 0.22) (0.00, 0.22) (0.46, 0.52) (0.00, 0.22) P value e 1.0000 0.9730 1.0000 1.0000 0.9730 1.0000 Candidate confirmed positive/total No. samples analyzed 0/144 70/144 144/144 0/144 70/144 144/144 Candidate confirmed POD (CC) 0.00 0.49 1.00 0.00 0.49 1.00 (0.00, 0.03) (0.40, 0.57) (0.97, 1.00) (0.00, 0.03) (0.40, 0.57) (0.97, 1.00) s r 0.00 0.51 0.00 0.00 0.51 0.00 (0.00, 0.16) (0.46, 0.52) (0.00, 0.16) (0.00, 0.16) (0.46, 0.52) (0.00, 0.16) s L 0.00 0.00 0.00 0.00 0.00 0.00 (0.00, 0.16) (0.00, 0.12) (0.00, 0.16) (0.00, 0.16) (0.00, 0.12) (0.00, 0.16) s R 0.00 0.51 0.00 0.00 0.51 0.00 (0.00, 0.22) (0.46, 0.52) (0.00, 0.22) (0.00, 0.22) (0.46, 0.52) (0.00, 0.22) P value 1.0000 0.9730 1.0000 1.0000 0.9730 1.0000 Positive reference samples/total No. samples analyzed 0/144 69/144 144/144 0/144 69/144 144/144 Reference POD 0.00 0.48 1.00 0.00 0.48 1.00 (0.00, 0.03) (0.39, 0.56) (0.97, 1.00) (0.00, 0.03) (0.39, 0.56) (0.97, 1.00) s r 0.00 0.51 0.00 0.00 0.51 0.00 (0.00, 0.16) (0.46, 0.52) (0.00, 0.16) (0.00, 0.16) (0.46, 0.52) (0.00, 0.16) s L 0.00 0.00 0.00 0.00 0.00 0.00 (0.00, 0.16) (0.00, 0.12) (0.00, 0.16) (0.00, 0.16) (0.00, 0.12) (0.00, 0.16) s R 0.00 0.51 0.00 0.00 0.51 0.00 (0.00, 0.22) (0.46, 0.52) (0.00, 0.22) (0.00, 0.22) (0.46, 0.52) (0.00, 0.22) P value 1.0000 0.9672 1.0000 1.0000 0.9672 1.0000 dLPOD (C vs R) 0.00 0.01 0.00 0.00 0.01 0.00 (–0.03, 0.03) (–0.10, 0.13) (–0.03, 0.03) (–0.03, 0.03) (–0.10, 0.13) (–0.03, 0.03) dLPOD (CP vs CC) 0.00 0.00 0.00 0.00 0.00 0.00 (–0.03, 0.03) (–0.12, 0.12) (–0.03, 0.03) (–0.03, 0.03) (–0.12, 0.12) (–0.03, 0.03) a  Results include 95% confidence intervals. b  Repeatability standard deviation.

c  Among-laboratory standard deviation. d  Reproducibility standard deviation. e P value = Homogeneity test of laboratory PODs.

( f )  Negative control solution.— 1×6 mL. Contains Tris- buffered saline (TBS; 150mmol/l) – Tween pH 7.6 + preservative. ( g )  Master lot entry ( MLE) card.— One card providing specifications for the factory master data required to calibrate the test: To read the MLE data, please refer to the Operator’s Manual. ( h )  Package insert .

purified and inactivated L. monocytogenes receptors + preservative + protein stabilizer. ( e )  Positive control solution.— 1×3 mL. Contains purified and inactivated L. monocytogenes antigen + preservative + protein stabilizer. 03/10/2019