5. AOACSPDSMethods-2018AwardsV3

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AOAC Official Method 2016.16 Curcuminoids in Turmeric Liquid Chromatography with UV-Vis Detection First Action 2016

(Applicable to the determination of curcumin, demethoxycurcumin and bisdemethoxycurcumin in turmeric ( Curcuma longa Linn) rhizomes, dietary ingredients and dietary supplement products containing turmeric alone or in combination with other dietary ingredients.)

Caution:

Solvents utilized are common-use solvents. Refer to adequate manuals or Material Safety Data Sheets (MSDS) to ensure that the safety guidelines are applied before using chemicals. Store in a flammable liquid storage cabinet. Harmful if inhaled, swallowed, or absorbed through the skin. Use appropriate personal protective equipment such as a laboratory coat, safety glasses, rubber gloves, and a fume hood. Dispose of all materials according to federal, state, and local regulations.

See Table 2016.16A for method performance data supporting acceptance of the method.

A. Principle

Samples are extracted with methanol using a wrist-action shaker. Dilution of highly concentrated samples may be required prior to LC analysis. Quantitation is performed with external calibration against reference standards for curcumin, demethoxycurcumin and bisdemethoxycurcumin.

B. Apparatus

( a ) Analytical balance.- Sensitive to at least 0.0001 g.

( b ) Grinder.- Retsch or equivalent centrifugal mill fitted with a 0.25 mm screen.

( c ) Volumetric pipet.- Class A, 25 mL.

( d ) Centrifuge tubes.- Conical polypropylene tubes, 50 mL.

( e ) Vortex mixer.

( f ) Centrifuge.- Benchtop with rotor for 50 mL conical tubes, maximum 21,000 x g .

( g ) Shaker.- Wrist action.

( h ) Micropipettors.- Capable of pipetting 200 and 1000 μL.

( i ) LC vials with caps.

( j ) Volumetric flasks.- Class A, 10 mL.