5. AOACSPDSMethods-2018AwardsV3

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1454 K oshy et al .: J ournal of AOAC I nternational V ol . 99, N o . 6, 2016

Table 7. Results from linearity of standards

Full range

Partial range

Residual,

Response factor, peak area/concn

Residual, %

r 2

% r 2

Compound

Concn, μg/mL

Mean peak area RSD r

, %

N

Withanoside IV

1321

5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5

9587357 4816148 2493821 1276177 651132 329761 166024 8721109 4335433 2241004 1147767 585013 297773 151167 21454279 14752690 8326374 4331341 2224593 1134003 14844246 9146389 4802093 2466826 1255825 13768071 13486375 7961571 4138065 2127011 1084444 12163116 12726145 7949510 4197521 2153855 1094892 634878 551256 573348 18055804

0.07 0.9999 −0.10

— —

— —

— —

661 330 165

0.07 0.10 0.33 0.13 0.11 0.01 0.16 0.21 0.42 0.33 0.11 0.08 0.34 0.09 0.28 0.35 0.12 0.07 0.17 0.08 0.29 0.41 0.08 0.06 0.11 0.09 0.29 0.39 0.08 0.13 0.05 0.12 0.32 0.36 0.09 0.08

−0.27

2.28 0.9999

7550 7727 7885 7987 8042 7466 7648 7796 7937 8058 — —

−0.31

2.40

1.16 0.90

83

−0.43 −6.50

41.3 20.6 1201

−0.66 −5.87

−18.28

Withanoside V

0.07 0.9999 0.08

— —

— —

600 300 150

−0.85

1.60 0.9999

−0.32

2.28 0.78

1.13 1.15

75

37.5 18.8 1475

−3.82

−0.75 −6.25

−13.37

— — —

— — —

— — —

Withaferin A

0.10 0.9633 −6.88

738 369 184

20.61 22.06

5.42 0.9999

23490 24130 24600 24876

−0.27

92

−19.39 −45.66 −67.23

1.18 0.46

46.1 23.1 1421 711 355 178 88.8 44.4 22.2 1382

−3.26

12-Deoxy withastramonolide

0.20 0.8942 −11.40

—

—

—

31.74 36.51

— —

— —

— —

8.42 0.9998

27031 27772 28276 28590

−0.30

−24.90 −53.75 −73.88

1.29 0.45

−3.47

Withanolide A

0.60 0.8120 −15.84

— — —

— — —

— — —

691 346 173

45.93 39.99

5.96 0.9998

23953 24625 25109 25528

−0.34

86

−29.31 −57.77 −76.50

1.55 0.19

43.2 21.6 1350

−3.56

Withanolide B

0.66 0.7602 −17.79

— — —

— — —

— — —

675 338 169

48.99 46.69

8.93 0.9998

24868 25520 25946 26203

−0.34

84

−29.75 −59.13 −77.74

1.55 0.20

42.2 21.1

552858

−3.63

— = data not included in partial range analysis