5. AOACSPDSMethods-2018AwardsV3

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The calculations used to determine the HorRat, a normalized performance parameter used to evaluate overall method precision, are provided below:

Table 2017.14. Gradient conditions for the separation of M. speciosa alkaloids Time, min Solvent A, % Solvent B, % Flow rate, mL/min 0 70 30 1.5 17 30 70 1.5 17.9 30 70 1.5 18 70 30 1.5

where SD(r) = population SD, represented by σ/n, where σ = sum of squares and n = number of replicates. Predicted RSD r (calculated, %): PRSD r = 2 C –0.15 where C = concentration of the analyte expressed as a mass fraction.

chromatogram; b 0

= y -intercept of the calibration curve for the

analyte; m 0 = slope of the calibration curve for mitragynine; V = volume of the test solution in milliliters; W = dry weight of the sample in grams; and D = dilution factor. Mitragynine from liquid samples was quantified in μg/mL using the following equation:

References: J. AOAC Int . 100 , 18(2017) DOI: 10.5740/jaoacint.16-0220 AOAC SMPR 2015.008 J. AOAC Int . 98 , 1106(2015)

where P 0 = peak area of the analyte in the sample chromatogram; 0 = y -intercept of the calibration curve for the target analyte; m 0 = slope of the calibration curve for mitargynine; and D = dilution factor. b

DOI: 10.5740/jaoac.int.SMPR2015.008

Posted: February 2018

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