5. AOACSPDSMethods-2018AwardsV3

8

6 K line et al .: J ournal of AOAC I nternational V ol . 100, N o . 3, 2017

Figure 3. Aloe-emodin region overlay HPLC chromatograms of (a) 20 ppb standard mixture, (b) solvent blank, (c) placebo of the powder product, (d) placebo of the liquid concentrate product, (e) 20 ppb standard mixture-spiked placebo of the powder product, and (f) 20 ppb standard-spiked placebo of the liquid concentrate product.

Table 3. Precision test for a typical lot of aloe concentrate product Lot No. Aloin A, ppb Aloin B, ppb Aloe-emodin, ppb 1 ND a ND ND 2 ND ND ND 3 ND ND ND 4 ND ND ND 5 ND ND ND 6 ND ND ND  Avg. NA b NA NA  RSD, % NA NA NA a  ND = None detected. b  NA = not available. Table 4. Precision test for a typical lot of aloe powder Lot No. Aloin A, ppb Aloin B, ppb Aloe-emodin, ppb 1 197.0 198.8 <20 2 198.7 202.7 <20 3 206.2 205.4 <20 4 201.4 206.6 <20 5 202.8 208.7 <20 6 201.3 207.7 <20  Avg. 201.2 205.0 NA a  RSD, % 1.6 1.8 NA a  NA = not available.

Table 1. Precision test for a typical lot of aloe vera 5× raw material Lot No. Aloin A, ppb Aloin B, ppb Aloe-emodin, ppb 1 ND a ND ND 2 ND ND ND 3 ND ND ND 4 ND ND ND 5 ND ND ND 6 ND ND ND  Avg. NA b NA NA  RSD, % NA NA NA a  ND = None detected. b  NA = not available. Table 2. Precision test for a typical lot of aloe vera 200× raw material Lot No. Aloin A, ppb Aloin B, ppb Aloe-emodin, ppb 1 345.9 325.5 22.9 2 355.1 351.5 26.6 3 340.6 327.9 23.7 4 349.1 330.1 22.1 5 348.8 330.1 24.1 6 341.4 333.9 21.6  Avg. 346.8 333.2 23.5  RSD, % 1.6 2.8 7.5