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each calibration curve to determine whether the points fall along a straight line. The calculated r 2 should be ≥0.995 for each analyte. (d) Test sample analysis .—Make single 5 μL injections of each test solution. F. Calculations The content of each phenolic compound in solid samples (mg/g) is determined using the following formula: where x = content of phenolic compound in the test sample in milligrams per gram, P 0 = observed peak area of the phenolic compound, b 0 = y -intercept of the calibration curve for phenolic standard, b 1 = slope of the calibration curve for phenolic standard, V = final volume of test sample preparation (mL), W = initial sample weight of test sample (mg), and D = dilution factor of sample preparation. Percent (w/w) can be calculated from milligrams per gram (mg) as follows:

The content of each phenolic compound in liquid samples (μg/mL) is determined using the following formula:

where x = concentration of phenolic compound in test sample (μg/mL), P 0 = observed peak area of phenolic compound, b 0 = y -intercept of the calibration curve for phenolic compound, b 1 = slope of the calibration curve for phenolic compound, V 1 = final volume of the test sample preparation (mL), V 0 = initial sample volume of the test sample (mL), and D = dilution factor of sample preparation. References: (1) Anal. Bioanl. Chem. 397 , 1883(2010) J. AOAC Int . (future issue) (First Action) AOAC SMPR 2017.015 J. AOAC Int . 101 , 315(2018) DOI: http://dx.doi.org/10.5740/jaoacint. SMPR2017.015 Posted: October 2018

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