6. AOACSPIFANMethods-2018Awards

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10 S alvati et al .: J ournal of AOAC I nternational V ol . 99, N o . 3, 2016

(3) AOAC SMPR 2015.004 (2015) J. AOAC Int. 98 , 1098. http:// dx.doi.org/10.5740/jaoac.int.SMPR2015.004 (4) AOAC SMPR 2015.005 (2015) J. AOAC Int. 98 , 1100. http:// dx.doi.org/10.5740/jaoac.int.SMPR2015.005 (5) Owen R. Fennema., Gregory, J.F. (1996) Food Chemistry , 3rd ed., CRC Press, Boca Raton, FL, pp 568–589 (6) Zhang, H., Chen, S., Liao, W., & Ren, Y. (2009) J. Food Agric. Environ. 7 , 88–93 (7) Determination of Water- and Fat-Soluble Vitamins by HPLC, Dionex Technical Note 89 (8) Zhang, J., Wang, Q., Kleintop, B., & Raglione, T. (2014) J. Pharm. Biomed. Anal. 98 , 247–252. doi:10.1016/j. jpba.2014.05.027 (9) Ndaw, S., Bergaentzlé, M., Aoudé-Werner, D., & Hasselmann, C. (2000) Food Chem. 71 , 129–138. doi:10.1016/ S0308-8146(00)00135-7

Conclusions

This enzymatic digestion LC-MS/MS method provides simultaneous quantitation of vitamins B 1 , B 2 , B 3 , and B 6 ; and was given First Action status for vitamins B 1 , B 2 , and B 6 . Method performance was demonstrated over 6 days in 14 different matrixes with three analysts and on two instruments. Intermediate precision averaged 3.9% and over-spike recovery was generally 95–105% for all four vitamins.

References

(1) AOAC SMPR 2015.002 (2015) J. AOAC Int. 98 , 1094. http:// dx.doi.org/10.5740/jaoac.int.SMPR2015.002 (2) AOAC SMPR 2015.003 (2015) J. AOAC Int. 98 , 1096. http:// dx.doi.org/10.5740/jaoac.int.SMPR2015.003