6. AOACSPIFANMethods-2018Awards

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Martin & Campos-Gim É

nez : J ournal of AOAC I nternational V ol. 98, N o. 6, 2015  1697

INFANT FORMULA AND ADULT NUTRITIONALS

Pantothenic Acid (Vitamin B 5 ) in Infant Formula and Adult/ Pediatric Nutritional Formula by Ultra-High Pressure Liquid Chromatography/Tandem Mass Spectrometry Method: Collaborative Study, Final Action 2012.16 Frederic Martin and Esther Campos-Giménez 1 Nestlé Research Center, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland Collaborators: A. Aoude-Werner, S. Bandhari, G. Jaudzems, I. Malaviole, M. Nixon, T. Norloos, L.T. Tanderup, S. Tennyson, C. Tool, M. Torres, P. van der Burgh, M. Vermeulen, C. Weihong, B. Wu, L.K. Yap

to synthetize coenzyme-A, which is needed in a vast range of biological roles, such as metabolism of fatty acids; it also plays a key part in the Krebs cycle. Historically, determination of PA was performed with a microbiological assay using Lactobacillus plantarum and its turbidimetric growth (1, 2). Even if this method is rather sensitive, its specificity is limited when dealing with complex food matrixes. More specific techniques have been tested for the analysis of this compound, such as indirect ELISA (3–5) and radioimmunoassay (6). Different LC methods have been developed as well, but the absence of a strong UV chromophore results in few methods using this detection available for the analyst. This detection difficulty can be circumvented by using highly selective MS. Andrieux et al. (7) used this approach in 2012, with a method combining a rapid sample preparation prior to the analysis of PA by ultra-high-pressure liquid chromatography (UHPLC) with a triple quadrupole MS detection. This method was proposed to the AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) and was approved as First Action AOAC 2012.16 method (8), with a recommendation to advance to a multilaboratory collaborative study. This paper presents the results of that collaborative study.

Received May 19, 2015. Accepted by SG June 13, 2015. 1 Corresponding author’s e-mail: esther.campos-gimenez@rdls. nestle.com The method was approved by the AOAC Official Methods Board as Final Action. See “Standards News,” (2014) Inside Laboratory Management , July/August issue. The AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) invites method users to provide feedback on the Final Action methods. Feedback from method users will help verify that the methods are fit for purpose and are critical to gaining global recognition and acceptance of the methods. Comments can be sent directly to the corresponding author. DOI: 10.5740/jaoacint.15-127 collaborative study participation (part 2). For part 1, each participating laboratory was asked to analyze two practice samples using the aforementioned method. Laboratories that provided results within a range of expected levels were qualified for part 2, during which each laboratory received 10 samples in blind duplicates. Results have been compared to the Standard Method Performance Requirement (SMPR ® ) 2012.009 established for pantothenic acid. Precision results (repeatability and reproducibility) were within the limits stated in the SMPR. Repeatability ranged from 1.3 to 3.3%, and reproducibility ranged from 4.1 to 7.0%. Horwitz ratio (HorRat) values were all <1, ranging from 0.33 to 0.69. The AOAC Expert Review Panel on Stakeholder Panel on Infant Formula and Adult Nutritionals Nutrient Methods determined that the data presented met the SMPR and recommended the method for Final Action status, which was then granted by the AOAC Official Methods Board. P antothenic acid (PA; vitamin B 5 ) is commonly present in foods of either plant or animal origin. This compound is an essential nutrient for humans, i.e., it is necessary In order to determine repeatability and reproducibility of AOAC First Action Method 2012.16 [Pantothenic Acid (Vitamin B 5 ) in Infant Formula and Adult/Pediatric Nutritional Formula by Ultra-High Pressure Liquid Chromatography/Tandem Mass Spectrometry], a collaborative study was organized. The study was divided in two parts: method setup and qualification of participants (part 1) and

Method

AOAC First Action Method 2012.16 was used, with minor modifications, mainly editorial.

AOAC Official Method 2012.16 Pantothenic Acid (Vitamin B 5 ) in Infant Formula and Adult/Pediatric Nutritional Formula Ultra-High Pressure LC/MS/MS Method

First Acction 2012 Final Action 2015

ISO–AOAC Method

(Applicable to the determination of free PA in infant formula and adult/pediatric nutritional formula.) Caution : Consult Material Safety Data Sheets prior to using chemicals and adhere to the safety precautions provided. Wear personal protective equipment when necessary.