6. AOACSPIFANMethods-2018Awards

268

Table 2017.03A Tryptophan SLV Data - Precision

Mean (g/100g, RTF)

SPIFAN Code a

No. of replicates b

Sample Type

RSDr

RSD iR

SRM1849a

CLC10-B

12

20.1

0.37

1.51

Infant Formula Powder PartiallyHydrolyzed Milk Based

410057652Z

12

25.3

0.56

0.97

Infant Formula Powder PartiallyHydrolyzed SoyBased

410457651Z

12

18.3

4.95

6.92

Toddler Formula Powder Milk-Based

4052755861

12

25.7

0.82

2.02

Infant Formula Powder Milk-Based

4044755861

12

24.1

0.2

1.82

Adult Nutritional Powder Low Fat

00859RF00

12

21.4

0.9

1.7

Child Formula Powder

00866RF00

12

23.7

0.94

1.33

Infant Elemental Powder

00795RF

12

32.2

0.24

1.56

Infant Formula Powder FOS/GOS Based

50350017W1

12

23.2

2

2.42

Infant Formula Powder Milk Based

K16NTAV

12

19.5

0.31

1.69

Infant Formula Powder SoyBased

E10NWZC

12

18.3

0.2

2.27

Infant Formula RTF Milk Based

EV4H2R

12

22.4

0.45

1.79

Adult Nutritional RTF High Prtotein

00730RF00

12

70.6

1.86

3.68

Adult Nutritional RTF High Fat

00729RF00

12

91.3

0.3

1.91

a Materials fromSPIFANSample Kit b Duplicates on each of 6 days, one laboratory

Table 2017.03B Tryptophan SLV Data – Accuracy

Native Trytophan Level (g/100g)

50%Overspike

100%Overspike

Number of replicates a

Sample Type

Spiking Material

Avg %Rec

SPIFAN Code

RSD, % Avg %Rec RSD, %

12

SRM1849a

CLC10-B

BSA

20.06

99.9

1.83

100.9

2.4

12

Infant Formula Powder PartiallyHydrolyzed Milk Based 410057652Z

BSA

25.28

104.3

3.3

104.1

2.71

11

Infant Formula Powder PartiallyHydrolyzed SoyBased

410457651Z

BSA

18.33

103.7

8.6

101.5

4.8

12

Adult Nutritional Powder Low Fat

00859RF00

BSA

21.39

96.2

5.2

99.5

3.9

10

Infant Elemental Powder

00795RF

BSA

32.22

100.7

2.5

102.4

2.7

12

Infant Formula Powder Milk Based

K16NTAV

BSA

19.51

100.5

2.99

101.7

1.99

12

Infant Formula Powder SoyBased

E10NWZC

BSA

18.27

93.8

5.09

99.5

3.53

12

Adult Nutritional RTF High Fat

00729RF00

BSA

91.3

104.8

1.6

104.9

1.3

a Samples spike in duplicate with 2 levels of spikes over 3 days. Samples with less than 12 replicates contained data points that were confirmed outliers

Table 2017.03C Tryptophan SLV Data - Linearity

Relative Calibration Error (%) Range (Low to High)

R 2

Experiment

Day1 Day2 Day3 Day4 Day5 Day6 Day7 Day8 Day9

0.99999 0.99994 0.99993 0.99996 0.99998 0.99996 0.99998 0.99997 1.00000 0.99999

-0.587 - 0.931 -4.569 - 0.519 -0.847 - 4.748 -1.165 - 0.956 -0.961 - 1.224 -0.658 - 3.395 -0.949 - 4.261 -0.476 - 3.158 -3.670 - 0.187 -0.947 - 0.850

Day10