6. AOACSPIFANMethods-2018Awards

40

L in et al .: J ournal of AOAC I nternational V ol . 100, N o . 1, 2017  151

Table 6. Representative chromatograms of tested SPIFAN matrices Matrix SPIFAN Code The data presented in this paper were submitted to the AOAC Expert Review Panel (ERP) for review at the AOAC Mid-Year Meeting held on March 16, 2016. The ERP determined that the Specificity . — Chromatograms from all of the SPIFAN matrixes helped to establish the specificity of this method for biotin. See Table 6 for representative chromatograms of tested SPIFAN matrixes. Ruggedness . — Several parameters were varied during validation to establish method ruggedness. Samples were prepared by three analysts and analyzed with C18 columns from four different lots. Fresh mobile phase, postcolumn reagents, intermediate standards, and working standards were made daily and used during validation. Conclusions

Table 5. Accuracy: recovery of SRM 1849a

Reference value, mg/kg powder

SRM product

Measured value, mg/kg powder

Recovery versus CoA, % a

Code

1849a CLC-10b

1.99

1.969 2.067 2.081 2.023 2.047 1.971 2.013 1.987 1.995

99

104 105 102 103 101 100 100 99

a  CoA = Certificate of Analysis.

Chromatogram

Child Formula Powder, placebo

00847RF00

Full-time range to include the riboflavin peak ( see below)

SRM 1849a

CLC10-b

Child Formula Powder

00866RF00

data presented here were in accordance with SMPR 2014.005 (5) approved by SPIFAN, and the method was granted First Action status.

(3) Lahély, S., Ndaw, S., Arella, F., & Hasselmann, C. (1999) Food Chem. 65 , 253–258. doi:10.1016/S0308-8146(98)00185-X (4) Höller, U., Wachter, F., Wehrli, C., & Fizet, C. (2006) J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 831 , 8–16. doi:10.1016/j.jchromb.2005.11.021 (5) Official Methods of Analysis (2016) 20th Ed., AOAC INTERNATIONAL, Rockville, MD, SMPR 2014.005. www.eoma.aoac.org

References

(1) Deborde, J.-L., & Joseph, G. (2015) J. AOAC. Int. 98 , 1044–1045, http://dx.doi.org/10.5740/jaoac.int.SMPR2014.005 (2) Thompson, L.B., Schmitz, D.J., & Pan, S.-J. (2006) J. AOAC Int. 89 , 1515–1518