6. AOACSPIFANMethods-2018Awards

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B runt et al .: J ournal of aoaC I nternatIonal V ol . 100, n o . 3, 2017 11

CCC used the sample Infant Formula Powder, Partially Hydrolyzed Milk-Based, Lot No. 410057652Z (Table 2016.14A , sample No. 7) for the determination of the LOD and LOQ by spiking it with a low level of fructan (Orafti P95, 0.046 g/100 g) and analyzing the sample 10 times on 2 different days. The LOD and LOQ were then calculated as

established values of the LOD and LOQ of both NRC and CCC are in good agreement and meet the SMPRs (5).

Precision

It was known that only a few samples in the SPIFAN sample kit contained fructan. To establish which samples contained fructan, both NRC and CCC analyzed the whole series of 19 samples independently from each other. In both laboratories, six of the 19 samples were found to contain fructans: (1) Sample No. 1, Child Formula Powder, Lot No. 00847RF00. ( 2 ) Sample No. 9, Toddler Formula Powder, Milk-Based, Lot No. 4052755861.

= × LOD 3 SD = × LOQ 10 SD

The LOD and LOQ results are summarized in Table 1 and also meet the requirements outlined in the SMPRs (5). The

Figure 3. Calibration curves using chitobiose as internal standard (I.S.; left column) and plots of the relative residuals (right column). (A) Fructose (Fru) at NRC; (B) glucose (Glc) at NRC; (C) fructose at CCC; and (D) glucose at CCC.