6. AOACSPIFANMethods-2018Awards

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Thompson et al.: J ournal of AOAC I nternational V ol. 98, N o. 6, 2015  1717

Table 3. Calibration standards and PLOQ

Na, mg/L Mg, mg/L P, mg/L K, mg/L Ca, mg/L Mn, mg/L Fe, mg/L Cu, mg/L Zn, mg/L Cr, μ g/L Se, μ g/L Mo, μ g/L

Cal Blk

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

Cal Std 1 Cal Std 2 Cal Std 3 Cal Std 4

0.500 0.200 0.500 1.00

1.00 0.00500 0.0500 0.00500 0.0200 0.800 0.400 0.800

2.50 10.0 20.0 0.25

1.00 4.00 8.00 0.10 0.50

2.50 10.0 20.0 0.25

5.00 20.0 40.0 0.50

5.00 0.0250 0.250 0.0250 0.100 4.00 20.0 0.100 1.00 0.100 0.400 16.0 40.0 0.200 2.00 0.200 0.800 32.0

2.00 8.00 16.0

4.00 16.0 32.0

PLOQ, mg/L a

0.50 0.0025 0.025 0.0025 0.010

0.4

0.2

0.4

0.013 c

PLOQ, mg/100 g b SMPRs, mg/100 g d

0.13 c

0.013 c

1.3

1.3

2.5

2.5

0.050

20 20

10 10

20 20

10

3

15

10

20

0.001 0.01 0.001

0.1

a  Units are µg/L for Cr, Mo, and Se. b  mg of Na, Mg, P, K, Ca, Mn, Fe, Cu, or Zn/100 g of reconstituted final product, or µg of Cr, Mo, or Se/kg of reconstituted final product for a typical dilution factor of 50:1.0 g RTF product or reconstituted powder/50 mL final volume. c  Note that the PLOQs for Mn, Fe, and Cu (in boldface) do not meet SMPR requirements. d  mg of Na, Mg, P, K, Ca, Mn, Fe, Cu, or Zn/100 g of reconstituted final product, or µg of Cr, Mo, or Se/kg of reconstituted final product.

Table 4. Summary of results for RSD ip in 11 SPIFAN matrixes tested in duplicate over 8 days (results were collected in terms of “per kg” as-is for powders in this case, rather than the default SPIFAN units of mg/100 g or μg/100 g reconstituted product)

Cr a

Se a

Mo a

Matrix/SPIFAN No.

Parameter

Na

Mg

P

K

Ca

Mn

Fe

Cu

Zn

A-RTF, high fat 00406RF00 b

Mean, mg/kg 1390 398 1010 2360 888 141 4.80 21.5 2.37 27.9 133 193 RSD, % 2.0 1.8 2.9 1.7 9.4 1.8 4.8 3.6 3.0 1.4 3.6 2.6 Mean, mg/kg 1020 330 951 1560 983 130 4.22 18.7 1.81 22.0 92.6 154 RSD, % 1.9 3.4 3.4 3.9 2.8 2.9 2.1 3.4 3.0 2.4 3.0 3.2 Mean, mg/kg 2270 430 2140 5810 2820 142 5.30 43.8 11.5 52.4 219 294 RSD, % 2.2 3.3 2.4 1.7 2.3 5.0 2.6 4.0 3.3 2.6 2.8 4.9 Mean, mg/kg 2070 1130 2710 5060 2740 428 14.0 59.8 6.34 62.1 268 565 RSD, % 2.8 2.9 1.8 2.1 2.7 1.3 2.7 3.5 3.3 2.6 3.2 3.0 Mean, mg/kg 1460 741 3490 5500 3790 271.9 7.62 57.7 5.15 37.6 213 261 RSD, % 2.6 2.5 2.0 2.5 1.8 1.3 1.4 3.2 3.7 1.7 2.9 5.1 Mean, mg/kg 2410 489 4560 7970 6310 215 4.67 105 7.20 64.6 209 160 RSD, % 3.0 3.3 2.4 3.1 2.2 6.3 1.3 1.9 1.7 2.1 2.8 7.5 Mean, mg/kg 1870 565 2800 6750 4690 44.3 1.05 116 5.43 65.9 230 159 RSD, % 3.2 2.9 1.7 3.3 2.5 7.7 2.0 2.0 4.9 1.5 3.8 8.2 Mean, mg/kg 1560 377 2310 6450 4190 21.4 1.05 83.7 4.90 43.4 239 184 RSD, % 1.8 2.6 4.4 1.1 2.2 20.2 2.6 3.1 3.9 2.6 2.7 4.0 Mean, mg/kg 2470 604 3950 7400 6480 53.6 2.30 111 4.88 49.2 242 297 RSD, % 2.9 2.9 3.6 2.2 2.2 9.2 2.6 2.5 3.9 1.8 4.1 3.6 Mean, mg/kg 2301.3 726 4250 7540 6470 72.5 3.31 114 5.40 73.9 223 328 RSD, % 2.4 2.8 5.0 2.6 3.5 5.2 2.3 2.4 4.1 2.3 3.3 4.5 Mean, mg/kg 179 59.4 298 1000 598 7.61 0.153 14.3 0.579 7.17 29.6 16.9 RSD, % 2.2 3.1 4.3 3.0 4.5 52.7 2.9 4.3 3.5 2.1 2.9 10.0

A-RTF, high protein 00414RF00

AP, milk protein 11750017V3 c

AP, low fat 00394RF00 PP 00412RF00 d IP, elemental 00403RF00 e IP, milk D04HTCVV

IP, milk, partially hydrolyzed 1172572116 IP, soy, partially hydrolyzed 117257651Z

I-RTF, soy E29JVLV f I-RTF, milk control

a  Concentrations in μg/kg. b  A-RTF = Adult ready-to-feed formula. c  AP = Adult powder formula. d  PP = Pediatric powder formula. e  IP = Infant powder formula. f  I-RTF = Infant ready-to-feed formula.