6. AOACSPIFANMethods-2018Awards

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Bidlack et al.: J ournal of AOAC I nternational V ol. 98, N o. 5, 2015  1389

product reconstitution in g (if necessary), S is the sample size in g, and P is the weight of product that is reconstituted in g (if necessary).

areas of the same standards injected before and after a set of samples have changed by more than 7%, the system was not equilibrated and the data are not acceptable. (c)  Calculation of trans vitamin K 1 standard concentration: T A A A ( ) 2 1 2 = + where T is the trans vitamin K 1 fraction, A 1 is the cis vitamin K 1 peak area, andA 2 is trans vitaminK 1 peak area. The  trans vitamin K 1 fraction is calculated for all standards, and the calculated trans vitamin K 1 fraction for all standards is averaged together (T a ) and used to calculate the trans vitamin K 1 concentration of all standards. (d)  Trans vitamin K 1 standard concentration: C C T ST S a = × where C ST is the concentration of trans vitamin K 1 in the working standard C S in µg/L and C s is the working standard concentration in µg/L. (e)  Preparation of standard curves .—For each working standard concentration, average the peak areas from each two consecutive sets of standards. Prepare a standard curve by performing a linear least-squares regression on trans concentration versus averaged peak areas. A standard curve must have an r 2 of 0.999 or better to be acceptable. (f)  Calculation of trans vitamin K 1 in a sample: = × × × 10.0 C C R S P p c where C p is the concentration of trans vitamin K 1 in µg/kg, C c is the concentration of trans vitamin K 1 in the injected sample determined from the standard curve in µg/L, 10.0 is the dilution volume of the sample in mL, R is the final dilution weight of a

G. Validation Data

See Tables 2 and 3.

Conclusions

AOAC 2015.09 met all of the SPIFAN SMPR and was approved for First Action status by an AOAC expert review panel at the AOAC Mid-Year Meeting in March 2015.

References

 (1) Ball, G.F.M. (1988) Fat-Soluble Vitamin Assays in Food Analysis A Comprehensive Review , Elsevier Applied Science, London, UK  (2) AOAC SMPR 2014.001 (2015) J. AOAC Int . 98 , 1036. http:// dx.doi.org/10.5740/jaoac.int.SMPR2014.001  (3) AOAC Official Method 992.27 (2012) Official Methods of Analysis of AOAC INTERNATIONAL , 19th Ed., Rockville, MD  (4) AOAC Official Method 999.15 (2012) Official Methods of Analysis of AOAC INTERNATIONAL , 19th Ed., Rockville, MD  (5) Schimpf, K.J., Thompson, L.B., & Schmitz, D.J. (2010) J. AOAC Int . 93 , 650–662  (6) Delmonte, P., Barrientos, S., & Rader, J.I. (2013) J. AOAC Int . 96 , 91–101. http://dx.doi.org/10.5740/jaoacint.12-191