AIMS Meeting Book_MYM2023

Click to edit Master title style PCR Legionella Implementation – Technical Challenges

Dead cells: v-PCR available, using of an easy, fast and safe reagent

For quantification: need of a Primary Standard. Stability in time ? Slighest variation in time How to confirm PCR results ? Compared to a non-optimized culture method

Technical Challenges

Dead cells

Viable but cannot form colonies on agar plates (VBNC)

Viable and can form colonies on agar plates

Culture ISO 11731

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