AIMS Meeting Book_MYM2023

Click to edit Master title style PCR Legionella Implementation – Regulatory Challenges

No correlation between CFU(culture) & GU (PCR) Comparison between the international ‘gold’ standard, the culture method, the ISO 11731 (but media non optimized) and a more specific, sensitive method, the PCR Easier to determine it for Legionella pneumophila than Legionella spp. Need of Primary Standard to calibrate the Quantification Standards New technology, digital PCR, can overcome those challenges : absolute quantification (no use of Quantification Standards) Recognition by national regulations as an official tool for routine analysis: slow-move but, with the increase, year after year of Legionella outbreaks and the global increase of temperature and humidity, awareness is rising.

Regulatory Challenges

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