AIMS Meeting Book_MYM2023

Challenges Facing Alternative Methods

 Acceptability of Alternative Methods?

 Industry is hesitant due to lack of data.

 CDC Guidance: • “(Alternative Methods)…should be validated against a standard method by a third party…”

• Need a framework for the most modern methods

 Challenges of alternative methods: • Drawing relationships between alternative method result units to CFU for Water Treatment Industry

• Differentiation between viable and non-viable cells

• Determining appropriate validation scheme when cultural reference method may not be consistently, quantitatively accurate.