AOAC 133rd Annual Meeting - Final Program

Scientific Sessions | Monday

SYMPOSIUM: Microbial Identification with Genomics and Proteomics in Food and Dietary Supplements 3:30 PM – 5:00 PM Plaza F Chairs: Quanyin Gao, Herbalife Manufacturing, LLC Peter Chang, Herbalife International of America Inc. Deborah McKenzie, AOAC INTERNATIONAL 3:35 PM Evolution, Trends, and Challenges in Microbial Test Methodologies in Food and Dietary Supplement Industry Deborah McKenzie, AOAC INTERNATIONAL Microbial methodology has long been one of the most important and used tools in the battle for food safety. Most notably used in preventative measures to ensure product safety, such method- ology have also been used to support nutrient label claims. The diversity of technology, diversity of commodities and matrices, diversity of technology employed, and even levels of valida- tion allow a plethora of options for both the food and dietary supplements industries. While the use of validated methodology for microbial contaminants in food and dietary supplement commodities is known, the advent of new commodities, new targets, emerging technologies, coupled with the need for testing time to result, have evolved traditional microbial methodology into a new generation methodological advances. However, these advances are not without challenges. A review of methods will outline the recent evolution and current trends for microbial methodology and the related challenges. 3:55 PM Microbiological Controls for Raw Material and Finished Product in Food and Dietary Supplement Industry Christopher Thompson, Quanyin Gao, Larry Engay, Jacqueline Tam, Herbalife Manufacturing, LLC, Peter Chang, Gary Swanson, Herbalife International of America Inc. The identification of microbial contaminants is an essential step in risk assessment and product disposition in the food and dietary supplement industries. Identification of a target microorganism relies on complex testing methods. Positive identification of zero-tolerance pathogens often requires identification to the strain, serovar and serotype level. As the sciences of genomics and proteomics develop, the methods to identify microorganisms are continuously improving. Biochemical identification techniques, while inexpensive and easy to perform, often lead to inconclusive results, and are limited in their ability to differentiate microorganisms. 16S rRNA sequencing can deliver genus and species identification but is dependent on a curated genome library and cannot differentiate further than the subspecies level.

The advancement of NGS and the availability of NGS in the quality control laboratory allows microbiologists to use multi-lo- cus strain typing and Whole Genome Sequencing to identify zero-tolerance pathogens, and to create an internal database of common non-harmful microorganisms that frequently occur in raw materials and the manufacturing environment. The addition of MALDI-TOF mass spectroscopy to the laboratory provides an inexpensive and simple method for the identification of strains, serovars and serotypes, so appropriate conclusions can be made regarding product disposition. 4:15 PM Genomics Identification in Microbial Tests (Next-Generation Sequencing) Amanda Manolis, Mario Gadanho, Tiina Karla, Thermo Fisher Scientific The use of DNA-based testing methods is increasing in the food protection industry. Molecular DNA testing methods are helpful tools for analysis of a variety of food and environmental prod- ucts that address some of the present industry concerns around organism identification. Several analytical methods have been proposed to answer the specific topic of species identification in food matrices. Next Generation Sequencing (NGS) has been introduced in recent years as a very powerful method for species identification in food products. This study is designed to optimize, evaluate and assess the utility the Ion Torrent™ technology (Ion Chef™ System and Ion GeneStudio™ S5 System, Thermo Fisher Scientific) for species characterization. 4:35 PM MALDI Biotyper: One System, One Workflow to Identify Bacteria, Yeasts and Molds within Minutes Daniele Sohier, Bruker Corporation The benchtop MALDI Biotyper identifies microorganisms using MALDI-TOF mass spectrometry to determine the unique proteomic fingerprint of a microorganism. The characteris- tic spectrum is used to reliably identify a microbial isolate by matching thousands of reference spectra. The MALDI Biotyper simplifies and shortens confirmation and identification steps, facilitating and harmonizing the workflow with only one system for bacteria, yeast and molds. The hands-on time per isolate is only 20 seconds for 95% of the microorganisms. The short time-to-result allows investigation of a full 96-spot target plate in 45 min. The MALDI Biotyper is recognized as an Official Method of Analysis by AOAC INTERNATIONAL with the two following reference numbers and claims: Method AOAC-OMA 2017.09: Confirmation and Identification of Salmonella spp., Cronobacter spp., Campylobacter spp., and other gram nega- tive organisms by the Bruker MALDI Biotyper method; Method AOAC-OMA 2017.010: Confirmation and Identification of Listeria Monocytogenes and Listeria spp., and other gram-posi- tive organisms by the Bruker MALDI Biotyper method. The MALDI Biotyper was awarded AOAC Method of the Year in 2018; the publication of the method OMA 2017.09 received the Best Manuscript Award in 2019. The MALDI Biotyper principle and workflow, the yearly Reference Library update, and validation studies will be presented.

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