AOAC 133rd Annual Meeting - Final Program
Poster Abstracts | Wednesday
evaluated in a multi-laboratory study as part of the AFNOR NF Validation certification process (NF102) following ISO 16140-2:2016 using unpaired test portions for one food matrix, reconstituted infant formula with probiotics. The candidate alter- native method was compared to the ISO 22964:2017 reference method. Sixteen participants from 15 laboratories throughout the European Union participated. Three levels of contamination were evaluated: 0 CFU/ target test portion, ~2 CFU/ target test portion) and ~10 CFU/ target test portion. The dLPODC values with 95% confidence interval were; 0.00 (-0.03, 0.03), -0.08 (-0.19, 0.02) and 0.00 (-0.03, 0.03) for the non-inoculated, low and high contamination levels, respectively. The dLPODC results indicate no significant difference between the candidate method and the reference method, or between presumptive and confirmed results for all three levels of contamination. Presenter: Ron Johnson, bioMérieux, Inc., Hazelwood, MO, USA, Email: ron.johnson@biomerieux.com P-W-092 John Mills , Deborah Briese , bioMérieux, Inc., Hazelwood, MO, USA A Comparative Evaluation of the GENE-UP ® Salmonella Assay for the Detection of Salmonella Species in Food Colors from Natural Sources The GENE-UP ® Salmonella spp. assay (SLM) is a qualitative assay based on real-time Polymerase Chain Reaction (PCR) for
the detection of Salmonella spp. in food products and environ- mental samples. For this study, the SLM assay was evaluated according to the current AOAC validation guidelines for Performance Tested Methods SM for the detection of Salmonella spp. in food colors from natural sources. The SLM method was compared to the FDA BAM Chapter 5: Salmonella . Samples were biochemically confirmed by both the traditional reference method procedures and by an alternative confirmation method. The alternative confirmation procedure included a direct streak to bioMérieux’s Chromogenic Salmonella Isolation Medium (ASAP) agar. The GENE-UP ® Salmonella method successfully detected Salmonella from all products evaluated. Using POD analysis, no statistically significant differences were observed between the number of positive samples detected by the candidate method and the reference method for all matrices. These data demon- strate that the GENE-UP ® SLM assay is a suitable rapid method for the routine testing of food colors from natural sources for the presence of Salmonella spp. Presenter: John Mills, bioMérieux, Inc., Hazelwood, MO, USA, Email: john.mills@biomerieux.com
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