AOAC 133rd Annual Meeting - Final Program

Scientific Sessions | Tuesday

SYMPOSIUM: How Can NGS Based Methods Advance Food Safety and Quality Programs? 10:15 AM – 11:45 AM Plaza F Chairs: Amanda Manolis, Thermo Fisher Scientific Jessica Williams, Thermo Fisher Scientific 10:15 AM Improving Pre-Enrichment Step for Microbiological Methods using NGS David Tomas Fornes, Lise Michot, Carine Blancpain, Nestec Ltd-Nestlé Research Center, Tiina Karla, Milja Tikkanen, Amanda Manolis, Thermo Fisher Scientific Pre-enrichment step is still required in order to detect low levels of Salmonella in foods despite the detection technology applied (e.g., cultural isolation, ELISA, molecular, etc…). During pre- enrichment, factors like Salmonella metabolic status, background flora and food ingredients may compromise the growth of stressed or injured Salmonella bacteria, producing false negatives. For microbiological methods optimization, pre-enrichment step is considered a “black-box”, with a very limited information about changes and interactions between microbial populations. In order to overcome this limitations and implement improve- ments during pre-enrichment, 16S NGS using Ion Chef™ Food Protection Instrument and Ion GeneStudio™ S5 Food Protection System (Thermo Fisher Scientific) was used to describe and monitor bacterial population during pre-enrichment in Buffered Peptone Water for Salmonella detection in challenging food items like spices, natural flavors and probiotics containing inhibi- tory compounds. The results showed not only Salmonella but also other bacte- rial species are inhibited during pre-enrichment producing false negative results. Information about changes in popula- tions allowed pre-enrichment broth optimization improving Salmonella detection in food matrices. 10:45 AM Isn’t Metagenomics Great Greg Jones, Roy Betts, Campden BRI Group, Mario Gadanho, Jani Holopainen, Thermo Fisher Scientific Food microbiology has always been heavily reliant on culturing organisms to answer questions on population dynamics. These investigations have led to greater understanding of microbial progression in food, however culture-based techniques have a series of biases. These biases are likely to have resulted in a partial understanding of the true nature of the complexity of the microflora of food. In recent years, microbiologists have been gifted the power of Next Generation Sequencing (NGS) as a tool to achieve a deeper understanding of the food microbiome. This presentation outlines the difference in attitude a microbiolo- gist must now assume in response to increased population data, as well as outlining some examples of how NGS based metage- nomics can help the food industry.

11:15 AM Metagenomics for Broad Spectrum of Safety Assessment in Food and Agriculture Nur Hasan, Brian Fanelli, Cosmos ID, Karen Jarvis, Christopher Grim, Padmini Ramachandran, Andrea Ottesen, U.S. Food and Drug Administration Next-Generation Sequencing has revolutionized microbiological sciences by revealing that virtually all environments, including food, soil, plant, animal and human body, are teeming with diverse microbial communities. It has become evident that the resident microbiota contributes biological functions that are essential to maintain healthy homeostasis. Conversely, disrupt- ing the healthy homeostasis can lead to dysbiosis and has been implicated with many diseases and pathologies. Studies will be showcased to demonstrate the power of high-throughput and high-resolution microbiome analysis as a potential tool for next generation diagnostic, safety assessment, and understanding the ecology of beneficial microbiome in food and agriculture. SYMPOSIUM: Global Perspectives on Mycotoxins in Food 3:00 PM – 4:30 PM Grand Ballroom 1 Chair: Kai Zhang, U.S. Food and Drug Administration

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