AOAC 133rd Annual Meeting - Final Program

Scientific Sessions | Tuesday

3:00 PM New Integrated Approaches to Tackle Global Rice Fraud Olivier Chevallier, Christopher Elliott, Queen’s University Belfast, LE Nguyen Doan Duy, Ho Chi Minh City University of Technology, Di Wu, Yangtze Delta Region Institute of Tsinghua University Zhejiang, Guangtao Zhang, Mars, Inc., Ernest Teye, University of Cape Coast Globalization of the food supply, and the resulting complex- ity in supply chains, has increased regulatory challenges and risks to consumers. Failure to characterize food quality, safety and authenticity has been shown not only to have devastating economic consequences, but also to create potential human health problems. Rice is the most important staple for more than half of the world’s population, and, because of the global economics of rice, it is a prime target for adulteration. The emer- gence and increasing prevalence of “plastic rice” coming from Southeast Asia is ringing alarm bells, as this fake rice presents a significant risk to human health. The approach currently devel- oped is employing a two-tiered system of testing technologies based on chemometric model libraries that provide “fingerprints” for thousands of rice samples. The system consist of a rapid screening method—based on field-deployable spectroscopic platform—that can be used in the field by producers, collectors, processors, dealers/traders, auditors, inspectors, and others to detect and semi-quantify a range of rice frauds, and a more sophisticated, laboratory-based approach employing LC-QTOF that can be used for detection, quantitation, and confirmation of the screening results for these same rice frauds. Latest progress on this work will be presented. 3:25 PM New Guidelines for Validation of Botanical Identification Methods James Harnly, U.S. Department of Agriculture, Peter Harrington, Ohio University, Holly Johnson, American Herbal Products Association, Adam J. Kuszak, National Institutes of Health Validated methods are necessary for authentication of botanical supplements and the ingredients that comprise a supplement. The guidelines for validation developed by AOAC in 2011 have proven to be cumbersome. New guidelines are possible that are conceptually simpler and much easier to implement. Basically, the guide consists of 5 basic steps: 1) assemble an inclusivity panel that represents a comprehensive sampling of the reference material and should always give a positive result and assemble an exclusivity panel that represents samples similar to the refer- ence samples and should always give a negative result, 2) select non-targeted fingerprinting method that is sensitive to as many of the chemical components of the sample as possible, 3) construct a model based on fingerprints of the reference samples, 4) set statistical limits a priori based on the purpose of the method, and 5) compare the unknown sample to the reference model and determine whether it is similar or not similar (inside or outside the statistical limits). Chemometric methods based on one-class modeling and the Q statistic (distance of the sample from the model) provide the simplest approach to modeling fingerprints and judging authenticity. Selection of the inclusivity panel consti- tutes the most difficult aspect of test.

will also touch upon why AOAC methods involving LC-FLD have been mostly employed for the quantitative analysis of aflatoxins and ochratoxin A. The presentation will subsequently highlight why the general LC-FLD-based approach is gradually shifting towards a direct analysis with faster and enhanced sensitivity using LC-MS/MS and automated workflows, integrating immu- noaffinity cleanup, and instrumental analysis. Besides, the recent efforts on implementation of multi-mycotoxin methods which were adopted to facilitate comprehensive risk assessments will also be presented. 4:10 PM Mycotoxin Testing Paradigm: Challenges and Opportunities for the Future Mark Sumarah, Justin B. Renaud, Agriculture and Agri-Food Canada, David Miller, Carleton University Mycotoxin contamination of agri-food and feed represents an important food safety challenge. Industry requires fast, reliable and inexpensive testing methods to help manage this problem. Fungal populations and their associated toxins have been impacted by climate change and changes in agricultural practice. These changes have resulted in the discovery of myco- toxins in areas where they had not previous been reported and new emerging mycotoxins such as NX toxin and 15ADON3G. LC-MS/MS is effective for mycotoxin analysis in a laboratory setting but is expensive, require highly qualified personnel and dedicated facilities and is not amendable for use on farm or at small to mid-sized processing facilities such as a grain elevator. Existing ‘on-site’ testing techniques mostly consist of antibody-based and spectrophotometric methods, both of which suffer from issues with cross reactivity and a lack of selectivity for the target analytes as discussed in our ‘Mycotoxin Testing Paradigm’. The development of innovative technologies such as miniaturized mass spectrometry, would allow for the acquisition of more information on mixtures of toxins present in a sample. This is especially important for feed products such as DDGS that are potentially contaminated with not only DON, its modified forms and additional toxins acquired during storage. SYMPOSIUM: New Tools for Food Fraud, an Old Problem with Perpetually New Intricacy 3:00 PM – 4:30 PM Grand Ballroom 2 Chairs: Jerry Zweigenbaum, Agilent Technologies, Inc. James Harnly, U.S. Department of Agriculture

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