AOAC 133rd Annual Meeting - Final Program

Poster ABSTRACTS

34 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL Determination of B Vitamins in Beverages by HPLC Water soluble B Vitamins are an essential group of organic compounds that are vital nutrients to maintain human health. They exist widely in human diet, but often don’t contain neces- sary amount to develop normal body functions. Therefore, vitamin-fortified beverages have grown in the food market. The U.S. Food and Drug Administration (FDA) mandatory requires manufactory to label the vitamins in their products. A reliable and simultaneous quantitative measurement of B vitamins is of increasing interest. This method describes an accurate and simple way for the simultaneous analysis of vitamins B1 (thia- mine), B2 (riboflavin), B3 (niacinamide), B5 (pantothenate), B6 (pyridoxine), and B9 (folic acid) in fortified energy drinks, liquid and powdered beverages by HPLC. The chromatography was optimized to allow for the best separation of aforementioned six vitamins, and to overcome potential matrix interferences. Accuracy of the method was evaluated and found to be within the corresponding certified value range by analysis of NIST SRM 3280. The precision of the method in analysis of vitamins B1, B2, MONDAY, SEPTEMBER 9, 2019 Poster Presentation Viewing: 10:00am–5:00pm Author Presentations: 12:00pm–1:00pm BOTANICALS AND DIETARY SUPPLEMENTS P-M-001 Xun Yan , AMWAY, Ada, MI, USA; Susie Burke , Shufang Nie , Nutrilite, Buena Park, CA, USA Anthocyanin Analysis and Stability Anthocyanins are widely present in berry extracts and are often used as quality indicating markers for nutrition products. The stability of anthocyanin is an important attribute in prod- uct design and development. In this study, we used aronia berry (chokeberry) extract as a model system and analyzed anthocyanin stability. The stability studies were set up at three temperatures, 25, 30, and 40°C. The samples were pulled at various time points and analyzed by pH differential colorimetric method for total anthocyanin content (AOAC 2005.02), and by a HPLC method for anthocyanin speciation. The stability data were analyzed and fit into a zero order degradation model. The activation energy and Q10 factor were derived from the model. These parameters are critical in predicting product shelf life using accelerated stability studies. Presenter: Xun Yan, AMWAY, Ada, MI, USA, Email: xun.yan@amway.com P-M-002 Ming Gao , Edward Rooney , Sneh Bhandari , Mérieux NutriSciences Corp., Crete, IL, USA

B3, B5, B6, and B9 in a variety of clear beverage matrices in terms of RSD% was found to be in the range of 0.9-11.6%. The method’s spike recovery of all targeted B vitamins was found to be in the range of 88.9-104% with the RSD% value 1.3-12.9%. LOQ of the method for analysis of vitamins B1, B2, B3, B5, B6, and B9 were calculated to be 1.63, 0.17, 0.17, 2.07, 0.68, and 0.77 mcg/mL, respectively. Presenter: Ming Gao, Mérieux NutriSciences Corp., Crete, IL, USA, Email: ming.gao@mxns.com P-M-003 Chad Nelson , Douglas Stevenson , Nu Skin Enterprises, Provo, UT, USA; Lesa Nelson , Genomic Reference Laboratories, Salt Lake City, UT, USA Genomic Strategies for Analyzing Botanical and Natural Products in Dietary Supplements Clear identification of species from botanical and other natural material is essential for today’s quality-control practices in nutri- tional supplement, pharmaceutical, and related natural-products industries. The total DNA content in a sample is a blueprint of all species present, distinguishing closely-related species, contaminants, and adulterants. Whole-genome sequencing (WGS) provides the complete DNA profile of a sample, both qualitative and relative abundance, which is extremely valuable information, particularly for complex natural products or mate- rials from less strictly-controlled sources. Genomic strategies for DNA testing of dietary supplements are presented, with focus on applications and benefits of WGS vs. other techniques, such as targeted panels or barcoding. Pros and cons of “non-targeted” vs. “targeted” methods are given, as well as schemes for routine monitoring of raw materials and finished products. WGS was applied to native, extracted, and final products to determine feasibility of successful DNA testing in the supply chain. DNA analysis of mushroom supplements ( Ophiocordyceps sinensis , Paecilomyces hepiali , and Ganoderma lucidum ) and various botanical products (e.g., green tea) were studied using different Next-Gen DNA sequencing approaches. For example, targeted analysis of P. hepiali , using an industry-standard ITS fungal panel, showed ambiguous species ID, while WGS resulted in clear conclusions for all fungal species present in this natural complex. Presenter: Chad Nelson, Nu Skin Enterprises, Provo, UT, USA, Email: cnelson@nuskin.com P-M-004 Ruth Muchiri , Richard van Breemen , Oregon State University, Corvallis, OR, USA Single Laboratory Validation of UHPLC-MS/MS Assays of Red Clover and Milk Thistle Dietary supplements are popular due to their historically known medicinal values. As an example, red clover ( Trifolium pretense