AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Monday

P-M-011 Dawn Randolph , Boris Nemzer , FutureCeuticals, Inc., Momence, IL, USA A Single-Laboratory Method Validation for Quantitation of Trigonelline in Whole Coffee Cherry Products by HPLC-DAD Trigonelline is a bitter alkaloid occurring in plants, mainly fenugreek ( Trigonella foenum-graecum ) which is where its name originates. Trigonelline is also found in whole coffee cherry (the fruit of the coffee plant) and green coffee beans, and, along with caffeine and total chlorogenic acids, trigonelline an inte- gral part of the phytonutrient profile of Coffeeberry™ products manufactured by FutureCeuticals (Momence, IL), such as coffee cherry powders, extracts, granules, and liquid concentrates. This test method was developed at FutureCeuticals as a means for screening both raw materials and finished Coffeeberry™ products. Trigonelline is extracted from samples using an ammonium formate buffer, separated by reversed phase HPLC with detection at 265 nm by PDA/DAD, and quantified using a commercially available standard. A single-laboratory validation study was performed on a whole coffee cherry extract and a commercially available powdered fenugreek product (Nature Vibe Botanicals), and the study included linearity, system preci- sion, method precision, intermediate precision, spike/accuracy, robustness, and ruggedness. The analytical range of the test method (0.1 to 5%) was determined to be fit for the purpose of quantitative analysis in raw ingredients, extracts, and finished blends containing extracts. Presenter: Dawn Randolph, FutureCeuticals, Inc., Momence, IL, USA, Email: drandolph@futureceuticals.com

P-M-012 Dawn Randolph , Boris Nemzer , FutureCeuticals, Inc., Momence, IL, USA A Single-Laboratory Method Validation for Quantitation of Total Glucosinolates in Brassica Vegetables by HPLC-DAD Glucosinolates are naturally occurring phytochemicals in Brassica vegetables and their extracts, which include broccoli, brussel sprouts, kale, mustard, cauliflower, and cabbage. In the presence of water, the myrosinase enzyme can hydrolyze the glucosinolate molecule, cleaving the glucose portion of the glucosinolate molecule, resulting in various nitriles, isothiocya- nates, and thiocyanates, including sulforaphane. This test method was developed at FutureCeuticals (Momence, IL) as a means for analyzing Brassica powders, sprouts, seeds, and extracts. In order to avoid the breakdown of glucosinolates during testing, samples are heat-inactivated to kill the activity of the myrosi- nase enzyme and extracted with 25 mM ammonium formate (pH 3.0). Glucosinolates are separated by HPLC and quanti- fied using glucoraphanin as the external standard. Response factors are used to quantify the glucosinolate compounds (glucoiberin, progoitrin, sinigrin, glucobarbarin, glucoerucin, and glucobrassicin) against glucoraphanin. A single-labora- tory validation study was performed on four Brassica matrices (broccoli sprout powder, kale powder, broccoli seeds, and mustard seed extract), and the study included linearity, system precision, method precision, intermediate precision, spike/accu- racy, robustness, and ruggedness. The analytical test method was determined to be fit for the purpose of analysis in Brassica powders, sprouts, seeds, extracts, and blends containing Brassica ingredients. Presenter: Dawn Randolph, FutureCeuticals, Inc., Momence, IL, USA, Email: drandolph@futureceuticals.com

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