AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

P-T-039 James Favell , Seamus Riordan-Short , Rob O’Brien , Matthew Noestheden , Supra Research and Development, Kelowna, BC, Canada Quantitation of 14 Cannabinoids in Edible Chocolate (SMPR ® 2017.019) Here we report single-laboratory validation data in support of SMPR ® 2017.019 for the quantitation of 14 cannabinoids in edible chocolates. Cannabinoids were extracted using heated ultrasonic extraction into acetonitrile, followed by cold stabi- lization to remove co-extracted waxes. Method performance metrics for 14 cannabinoids, including recovery, repeatability and intermediate precision, were evaluated in dark chocolate truffles, milk, white and dark chocolate. The developed extraction procedure is amenable to UV or MS-based detectors. Presenter: Matthew Noestheden, Supra Research and Development, Kelowna, BC, Canada, Email: matt@suprarnd.ca P-T-040 Ryan Hayward , T. Nguyen , Rob O’Brien , Matthew Noestheden , Supra Research and Development, Kelowna, BC, Canada Quantitation of Terpenoids in Plant Materials Using High-Temperature Headspace Sampling Plants are the main source of many high-value bioactive terpenoids used in the medical, fragrance and food industries. Increasing demand for bioactive plants (e.g., cannabis and extracts thereof) require robust authentication approaches to verify feedstock, identify product adulteration and ensure product safety for consumers. Reported here are single-labora- tory validation details for a robust testing method to quantitate terpenes in plant materials using high-temperature headspace (180°C) gas chromatography-mass spectrometry. This approach not only effectively quantifies the terpenoids present, but the optimized temperature corresponds to the temperatures used in vaporizers and e-juice devices. As a result, the method scope was expanded to include e-liquid/e-juice products to profile the range of potentially bioactive terpenoids delivered to users of such devices. Presenter: Ryan Hayward, Supra Research and Development, Kelowna, BC, Canada, Email: ryan@suprarnd.ca P-T-041 David Alonso , Joe Binkley , LECO Corp., St. Joseph, MI, USA; Gail Harkey , LECO Corp., Fairburn, SD, USA Streamlining Cannabis Testing Using Comprehensive Two-Dimensional Gas Chromatography with Time-of- Flight Mass Spectrometry (GCxGC-TOFMS) Composition of cannabis is very important in determining its utility, potency and medicinal effectiveness. Unfortunately, many cannabis products for medical and recreational use have been tested for potency, but not for contaminants and thus pose a significant safety risk to users. This is in part due to the fact that cannabis analysis may require tedious sample preparation followed by data collection using different instrumental tech- niques depending on the desired outcome (e.g., terpene profiles, residual solvent determination, potency, etc.). In this study, more

efficient sampling techniques together with enhanced chromato- graphic resolution and high performance mass spectrometry were employed to perform comprehensive cannabis analysis in a minimal amount of steps. Presenter: Gail Harkey, LECO Corp., Fairburn, SD, USA, Email: gail_harkey@leco.com P-T-042 Walter Wilson , Melissa Phillips , Catherine Rimmer , National Institute of Standards and Technology, Gaithersburg, MD, USA Future Plans at the National Institute of Standards and Technology for a Hemp Quality Assurance Program and Reference Materials Similar to the NIST Health Assessment Measurements Quality Assurance Programs (QAPs) for the Dietary Supplement community (https://www.nist.gov/programs-projects/ health-assessment-measurements-quality-assurance-program), we are developing a Hemp QAP to improve the comparability of the hemp measurements for testing laboratories and prod- uct manufacturers, while supporting the development of new reference materials (RMs). The work presented in this poster will provide a summary of NIST future plans. Briefly, NIST intends to acquire, process, and package a hemp dried plant material. This material will be characterized at NIST for cannabinoids and value assigned for total THC content using multiple meth- ods (both LC and GC approaches) to include determination of Δ 9 -THC and THCA individually. The material will also be screened for heavy metals, mycotoxins, and pesticides at NIST, and values assigned as appropriate. Screening for total yeast, mold, and microbiological contaminants will conducted by a trusted third-party laboratory. The material will also be included in a hemp QAP interlaboratory comparison for THC and other cannabinoids, heavy metals, mycotoxins, and pesticides based on the results of NIST analyses, and will be made available for sale as an RM as soon as possible upon conclusion of the work. NIST will also begin to evaluate the options for the acquisi- tion of a hemp oil for future interlaboratory studies and RM development. Presenter: Walter Wilson, National Institute of Standards and Technology, Gaithersburg, MD, USA, Email: walter.wilson@nist.gov P-T-043 Carly Barone , Ellis Wright , Thomas Grace , Bia Diagnostics LLC, Colchester, VT, USA Quantitative Determination of Terpenes from Different Parts of the Cannabis sativa L. (Hemp) Plant Using Headspace/Gas Chromatography/Mass Spectrometry Cannabis sativa L. (Hemp) biproducts are being marketed as having beneficial therapeutic properties for various ailments. Volatile terpenes are the largest group of Cannabis sativa L. (Hemp) compounds and are mainly responsible for the distinct aroma of C. sativa strains. Additionally, they, contribute to the “entourage effect” of C. sativa . Many studies have been focused on cannabinoids, while terpenes, an essential part of the hemp industry, have not been heavily researched. This study sampled multiple plants to investigate the quantity of terpenes

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