AOAC 133rd Annual Meeting - Final Program

Scientific Sessions | Monday

1:55 PM Arsenic, Iodine, and Bromine Speciation Analysis in Infant Formula, and Nutritional Products using HPLC-ICP-MS Lawrence Pacquette, Abbott Laboratories, Jenny Nelson, Shuofei Dong, Michiko Yamanda, Agilent Technologies, Inc., Courtney Tanabe, University of California Elemental speciation plays an important role in food safety. Therefore, government regulations are focusing on stringent testing of various elemental species in food products. In addi- tion, there is growing consumer concern for the presence and levels of various elemental species especially in infant formula and nutritional products. Using high-performance liquid chro- matography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS), species of iodine (I − , IO 3 − ), bromine (Br − , BrO 3 − ), and arsenic [As(III), As(V), DMA, MMA] were separated and analyzed by two methods in infant formula, nutritional products, and NIST SRMs. The halogen species were separated in less than 6.5 minutes by one method and the arsenic species were separated in 12 minutes by another method. The limit of quanti- tation for I − , IO 3 − , Br − , and BrO 3 − were between 0.2 µg/kg and 3 µg/kg, and for As(III), As(V), DMA, and MMA between 2.5 µg/kg and 5 µg/kg. Concentrations of the halogen and arsenic species in products and NIST SRMs ranged from 0 - 25660 µg/ kg and 0 - 60 µg/kg, respectively. Also, spike sample recover- ies of 20 µg/kg and 40 µg/kg halogen species ranged from 87% to 102%, and spike sample recoveries of 5 µg/kg arsenic species ranged from 93% to 109%. 2:15 PM Analysis of Thyroid Hormones in Dog Food by LC-ICP-MS Robert Wilson, Enrique Yanes, Jana L. Brueggemeyer, U.S. Food and Drug Administration The thyroid hormones triiodothyronine (T3) and thyroxine (T4) are synthesized in the thyroid gland from the hormone precur- sors monoiodotyrosine (MIT) and diiodotyrosine (DIT). These hormones play an essential role in regulating cellular activity, growth, and brain development. However, excessive concentra- tions of T4 or T3 from either overproduction by the thyroid gland or ingestion of exogenous thyroid hormones can lead to thyro- toxicosis. Continued exposure to excessive hormone levels can cause damage to the heart and result in serious health issues. Recently, several incidences of thyrotoxicosis in dogs suspected to be caused by the consumption of thyroid hormone contami- nated pet foods and treats have been identified. However, at the time a suitable method for the quantitation of T3 and T4 in pet foods and treats was not available. In this work we developed and validated a method for the quantitation of iodide, MIT, DIT, T3, and T4 in dog food by high-performance liquid chroma- tography with inductively coupled plasma mass spectrometry detection (LC-ICP-MS) following an enzymatic digestion. The optimized method was used for several studies involving commercially available dog food products and for the analysis of multiple dog food and treat consumer complaint samples which resulted in several product recalls.

2:40 PM Heavy Metal Contaminants from Cannabis Vaporizer Cartridges: Valid Concern or Blowing Smoke? Ini Afia, CannaSafe, Robert Weltman, University of California, Kyle Boyar, Medicinal Genomics The State of California’s roll out of heavy metal testing on January 1, 2019 sent cannabis vape cartridge manufacturers into a state of panic when many quickly realized that the hard- ware being used would not pass Phase III compliance testing. In the absence of rigorous study, this spurred a hailstorm of media stories that suggested heavy metal contamination of canna- bis vaporizer cartridges poses serious health concerns for the general public. These media scares are potentially damaging for the reputation of cannabis vaporizer cartridges, especially when sample preparations currently being employed are not standard- ized to evaluate the contribution of the vaporizer cartridge to total metal exposure. In this pilot study, a robust sample analysis method for testing the contribution of a vaporizer cartridge to four toxicant metals (Pb, Hg, Cd, As) in the aerosol. Three brands of cartridge hardware using standardized settings were tested. Testing the canna- bis-based formulations in the cartridge, and air-sampling pump to create realistic cannabis aerosols, we assessed how much of these heavy metals would be aerosolized and consumed. Preliminary data suggests that heavy metals do leach from the hardware and a significant portion of these metals end up in the condensate captured. ROUNDTABLE: Method Fitness in a time of FSMA—How Should Laboratories and Food Manufacturers Decide on Method Suitability? 1:30 PM – 3:00 PM Plaza F Chairs: David Legan, Eurofins Larry Cohen, Treehouse Foods, Inc. Christie Hancock, Conagra Brands Gretchen Gutierrez, Matrix Sciences Felix Haesler, Eurofins GeneScan Technologies GmbH DeAnn Benesh, 3M W. Evan Chaney, Diamond V/Cargill Animal Nutrition The Food Safety Modernization Act (FSMA) requires food companies to verify that their preventive controls are effective. To do this they test product and environmental samples. FSMA additionally requires that methods be scientifically suitable, or fit-for-purpose, and this responsibility also lands on the food company. There is very little guidance for manufacturers or commercial laboratories that defines “fitness for purpose” in practical terms. This is different from the situation for regulatory laboratories and diagnostic test kit makers who have detailed validation schemes and guidance documents available. The panelists at this roundtable will describe the current state of affairs, and the discussion will permit clarification and allow ideas to be shared that might lead to improved approaches.

WWW.AOAC.ORG 301.924.7077 8

8 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL