AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Wednesday

P-W-020-A Manami Kobayashi , Shimadzu Japan, Kanagawa, Japan; Miho Kawashima , Yusuke Inohana , Yoshihiro Hayakawa , Shimadzu Japan, Kyoto, Japan; Nozomi Maeshima , Junichi Masuda , Shimadzu Japan, Kanagawa, Japan; Alan Owens , Shimadzu USA, Columbia, MD, USA Highly Sensitive Direct Analysis of Glyphosate, Glufosinate, and AMPA in the Beverages by LC-MS/MS Glyphosate and glufosinate are active ingredients in widely used herbicides. It is well-known that glyphosate is degraded into aminomethylphosphonic acid (AMPA) as a metabolite in soil and water. The required LLOQ for glyphosate and AMPA in water is 0.1 ug/L (ng/mL) in the EU. Since glyphosate, glufos- inate and AMPA are highly polar compounds, their retention on the reversed phase column are weak. Therefore, a derivatization method with such as FMOC is performed for these compounds. To reduce the complex and time-consuming derivatization, we introduce a high-sensitivity direct analysis of glyphosate, glufos- inate and AMPA without derivatization. With limited pretreatment procedures such as filtering and dilution, highly sensitive results could be obtained with good recovery factors for vary of bever- ages. This poster describes the development of a direct analysis method by LC-MS/MS for glyphosate, glufosinate and AMPA in beverages. Presenter: Alan Owens, Shimadzu USA, Columbia, MD, USA, Email: amowens@shimadzu.com P-W-020-B Uwe Oppermann , Shimadzu Europa GmbH, Duisburg, Germany; Erich Leitner , TU Graz, Graz, Austria Determination of Mineral Oil Hydrocarbons in Food and Food Packaging Using the LC-GC Online Technique Mineral oil components such as mineral oil saturated hydrocar- bons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) can be identified almost everywhere in the environment in fatty components in cosmetics, food and transport packaging mate- rials. Both compounds, the MOSH and also the MOAH are easily absorbed by the human body from food, and can accu- mulate in body fat and in the organs. Yet, it cannot be excluded that MOAH fractions may contain carcinogenic compounds. That´s why European Community (EC) is under pressure for fixing analytical determination limits of MOSH and MOAH in food and food packaging. Because of their complexity it is not possible to resolve MOH mixtures into individual components for quantification. However, it is possible to quantify the concen- tration of total MOSH and MOAH fractions, as well as certain sub-classes, using methods based on gas chromatography (GC). The system combines HPLC (LC-20ADXR) and GC (GC-2030) technology with flame ionization detection (FID) for a highly efficient analysis covering preparation, pre-separation and automated processes. The Shimadzu MOSH/MOAH analyzer has been designed specifically for sensitive and fast detection of mineral oil contaminations based on the European Norm DIN EN 16995:2017: Determination of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) with online HPLC-GC-FID Analysis. Presenter: Uwe Oppermann, Shimadzu Europa GmbH, Duisburg, Germany, Email: uo@shimadzu.eu

P-W-021 Stefania Squadrone , Paola Brizio , Caterina Stella , Sabina Pederiva , Angelo Ferrari , Maria Cesarina Abete , Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, CReAA, National Reference Centre for the Surveillance and Monitoring of Animal Feed, Turin, Italy Rare-Earth Elements in Food of Terrestrial Origin Rare-Earth Elements (REEs) are not that rare in nature, being the 15th most abundant components of the earth’s crust. Due to their unique physical and chemical properties, they are essential in a variety of technologies worldwide. REEs are contaminants of emerging concern, but they have been scarcely investigated yet and remain still unregulated, not being part of environmen- tal or safety monitoring plans. The concentrations of REEs were determined by ICP-MS in samples of fruit, plant vegetables, leguminous and mushrooms from Italy to evaluate the human/ animal exposure through their consumption. The sum of REE (SREES) were found in the range 4.4 – 81 mg Kg -1 with the following decreasing order: leguminous (seeds) > mushrooms > plant vegetables > fruit. Leguminous have the highest ability to accumulate REE from the environment while in fruit values were almost negligible. The mean concentration of the single REE was Sc (scandium) > Ce (cerium) > La (lanthanum) > Nd (neodym- ium) >Y (yttrium) > Pr (praseodymium) > Sm (samarium) > Gd (gadolinium) > Dy (dysprosium) > Eu (europium) > Er (erbium) > Yb (ytterbium) >Tb (terbium) > Ho (holmium) >Tm (thulium) > Lu (lutetium). Values found in our study were in the range or lower than those found from other parts of the world in the same matrices (e.g., Spalla et al., 2009; Li et al., 2002; Falandysz et al., 2017, Zhuang et al., 2017) and comparable to a previous investigation on plant feed and fruit (Squadrone et al. 2019). Presenter: Sabina Pederiva, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, CReAA, National Reference Centre for the Surveillance and Monitoring of Animal Feed, Turin, Italy, Email: sabina.pederiva@izsto.it P-W-022 Emiliano De Dominicis , Orianna Marcon , Andrea Vittadello , Enrico Nieddu , Silvia Carnio , Mérieux NutriSciences Corp., Resana, Italy; John Szpylka , Mérieux NutriSciences Corp., Crete, IL, USA; Eric Gordon , Mérieux NutriSciences Corp., Chicago, IL, USA; Samim Saner , Mérieux NutriSciences Corp., Istanbul, Turkey Coffee Integrity and Non-Intentional Added Substances (NIAS)—Case Study of Mass Spectral Identification of Tetrahydrofuran as a Degradation Product of 1,4-Butanediol Contained in Compostable Coffee Capsules COFFEE SAFETY: Are contaminants present in the product? If so, which? Have pesticides been used during cultivation? Are there residues of toxins, mineral oils, PFAS etc. deriving from an unsuitable management during collection, storage and transport? Has the packaging material been tested to avoid the presence of NIAS contaminants in the packaging? COFFEE QUALITY: How much caffeine is contained in my coffee? Are nutritional and composition parameters compliant? Are there any residues of detergents used in production plants? Do we know the actual satisfaction of consumers and where the product

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