AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Wednesday

matched calibration methods were applied, compared, and evaluated in terms of recovery, efficiency, advantages, and limitations. Presenter: Joseph Konschnik, Restek Corp., Bellefonte, PA, USA, Email: joe.konschnik@restek.com P-W-026 Emiliano De Dominicis , Cristina Bonato , Michela Casarotto , Giampaolo Perinello , Mérieux NutriSciences Corp., Resana, Italy; Paolo Matteini , Mérieux NutriSciences Corp., Prato, Italy; Samim Saner , Mérieux NutriSciences Corp., Istanbul, Turkey; Pierre Metra , Mérieux NutriSciences Corp., Paris, France; Karen Mandy , Mérieux NutriSciences Corp., Cedex, France; John Szpylka , Mérieux NutriSciences Corp., Crete, IL, USA Optimization, Validation and Accreditation of GC- MS/MS Method for MCPDs and Glycidyl Esters Determination in Baby Food and Infant Formula On the basis of the most recent legislative and toxicological indications, an effective and suitable GC-MS/MS method was optimized, validated and accredited for the determination of MCPDs and glycidyl esters in baby foods and infant formula. This method utilizes SPE Purification followed by glycidol group bromination, acidic hydrolysis of esters, and PBA derivatiza- tion. Analysis is achieved by GC-EI-MS/MS (MRM mode). The high level of standardization allows a constant and widespread control of all critical and potentially critical points through the use of non-isotopically labeled standard ester forms (Recovery%), isotopically labeled internal standard free forms (Matrix Effect% and RSD%), and CPS (Batches Verification). Proficiency Testing is also performed for accuracy assessment. In wet foods, LODs of 1 ppb were realized for 2-MCPD esters, 3-MCPD esters, and glycidyl esters reported as 2-MCPD in fat, 3-MCPD in fat, and glycidol in fat, respectively. All authors thank Danone and Mérieux NutriSciences for the opportunity to carry out these activities of research, development, validation, and accredi- tation, certainly important for the dissemination of analytical services that are really performing in terms of food safety, especially for the most sensitive part of the population such as children and babies. Presenter: John Szpylka, Mérieux NutriSciences Corp., Crete, IL, USA, Email: john.szpylka@mxns.com P-W-027 Xuesong Zhang , Zhu Wang , National Institute for Nutrition and Health, The Chinese Center for Disease Control and Prevention, Beijing, China; Yufei Wang , Bao’an Central Hospital of Shenzhen, Shenzhen, China Establishment of the Liquid Chromatographic/ Fluorometric Method for Determination of Alkyresorcinols in Whole Grain To determine the quality of whole wheat products, it is neces- sary to establish a method for determination of alkylresorcinols (ARs) which are the markers of whole wheat, black wheat and other cereal products. Taking wheat and black wheat as main

research objects, factorial design was used to investigate the effect of extraction reagents, extraction methods, and sample status on the determination of ARs. And ARs detection conditions was established by comparing chromatographic conditions and scanning fluorescence wavelengths. Taking into account the toxicity of the reagents and efficiency, it was better that the comminuted sample was ultrasonically extracted with ethanol for 30 min before loading on the Waters CORTES-C18 column with ethanol: acetonitrile (30:70, v:v). The fluorescence detector was set at a wavelength of 272 nm for excitation and 296 nm for emission to detect ARs. The linear range was 0.050-10.0 μg/ ml and the coefficient R2 ≥ 0.9999. RSD of precision experi- ment was less than 5% and the spiked recovery rates of three levels were 94.5-104%. The total amount of ARs was 47.9-54.3 mg/100 g in commercially available wheat and 53.6-60.9 mg/100 g in black wheat. The liquid-fluorescence method established in this study can effectively and rapidly separate ARs from wheat and black wheat and has the advantages of simple operation, high sensitivity, and accuracy. Presenter: Xuesong Zhang, National Institute for Nutrition and Health, The Chinese Center for Disease Control and Prevention, Beijing, China, Email: xuesong_zhang0729@163.com P-W-028 Uwe Oppermann , Johannes Hesper , Jan Knoop , Shimadzu Europa GmbH, Duisburg, Germany Analysis of Contaminants in Beverages Using ICP-Mass Spectrometry Beer is the most popular alcoholic beverage in Europe and enjoys a particularly high status due to the German Beer Purity Law of 1516 (the “Reinheitsgebot”), which uniquely defines the ingredients of beer to be hop, malt, yeast and water and makes the German Beer Purity Law the oldest food law in the world. Statistically, per capita beer consumption in some European countries was more than 100 liters in 2017. For these levels of consumption, the question arises: just how healthy is beer and what does beer contain? Beer contains hops, malt, yeast and water, as well as all major B vitamins, bitter substances, minerals and trace elements (e.g. Ca, Na, Mg and Zn) that are important for human nutrition. However, undesirable substances such as pesticides and heavy metals are also found. For simul- taneous multi-element analysis at ultra-low levels such as As, Se and Sb the inductively coupled plasma mass spectrometer ICPMS-2030 has been used. In this study commercially avail- able beverages such as beer and wine have been measured. Thanks to the ICPMS-2030 system, analysis of beer could be performed without any time consuming sample preparation. All analyzed samples are only degassed with ultra sonication. After this treatment they are acidified with nitric acid and diluted with deionized water–then directly aspirated to nebulization system of the ICPMS-2030. 7 different elements are simultaneously quantified: Analytical results for a variety of beers are presented. Presenter: Uwe Oppermann, Shimadzu Europa GmbH, Duisburg, Germany, Email: uo@shimadzu.eu

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