AOAC 2018 Methods

Table 2018.03A : GC-MS/MS transition reactions of the derivatives of free 3-MCPD, 2-MCPD and 3- MBPD (and their corresponding IS), used to monitor 3-MCPD (free and bound), 2-MCPD (free and bound) and glycidyl esters after conversion in 3-MBPD ( quantifier ions are indicated in bold). Compound Name Precursor ion (m/z) Product ion (m/z) Dwell time (ms) Collision Energy (V) Time segment 3-MCPD 196 147.1 25 5 1 147 91 25 20 1

150 201 201 196 198 196 201 201 240 147 240 150 245 245

93

25 25 25 25 25 25 25 25 40 40 40 40 40 40

20

1 1 1

3-MCPD IS

149.2

5

92.1 104 104

25 15 15 10 30 10 15 30 15 15 35 5

1, 2 1, 2 1, 2 1, 2 1, 2

2-MCPD

91

104.1

2-MCPD IS

106

147.1

2 2 2 2 2 2

3-MBPD

91

91.1

93

3-MBPD IS

149.1

92.1