AOAC 2018 Methods

Table 2018.03B. Pipetting schema for preparation of MCPD and glycidyl esters working solutions. Figures in italic and bold are calculated with a target concentration of individual stock standard solution (F) and must be calculated according to exact concentrations. Total Volume (mL) PP-3- MCPD PP-2- MCPD Gly-P PP-3- MCPD- d5 PP-2- MCPD- d5 Gly-P-d5 Stock standard solution target concentration (expressed ester eq.) in μg/ml - 1000 1000 1000 1000 1000 1000 Molecular Weight (Da) - 587.36 587.36 312.49 592.39 592.39 317.52

Stock standard solution (expressed as free form eq.) μg/ml Molecular Weight (Da)

-

188.20 188.20 237.06 195.04 195.04 249.06

-

110.54 110.54

74.08

115.54 115.54

79.08

Mix ISTD (1 µg/ml) Mix cal 1 (5 µg/ml)

Volume (µL) of individual stock solution Volume (µL) of individual stock solution

102.5 µL 102.5 µL 80.3 µL

20 mL

-

-

-

20 mL 532 µL 532 µL

422 µL

-

-

-

Mix cal 2 (0.1 µg/ml)

Volume (µL) of Mix cal 1 20 mL

400 µL of Mix cal 1