AOAC 2018 Methods

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Table 2018.03C: Preparation of the calibration curve for bound glycidol, 2-MCPD and 3-MCPD. Volume to be pipetted from, [µL] Cal 0 Cal 1 Cal 2 Cal 3 Cal 4 Cal 5 Cal 6 Cal 7 PP-3-MCPD, PP-2-MCPD and Gly-P (5 µg/ml) Mix Cal I 0 0 0 0 0 20 60 200 PP-3-MCPD, PP-2-MCPD and Gly-P (0.1 µg/ml) Mix Cal II 0 20 50 100 300 0 0 0 d5- PP-3-MCPD, d5-PP-2- MCPD and d5-Gly-P (1 µg/ml) Mix ISTD 50 50 50 50 50 50 50 50

Equivalent to (µg in vial)

Cal 0

Cal 1

Cal 2

Cal 3

Cal 4

Cal 5

Cal 6

Cal 7

2- & 3 - MCPD & Glycidol

0.002

0.005

0.01

0.03

0.1

0.3

1.00

2- & 3 -MCPD- d5 & Glycidol-d5

0.05

Equivalent to (mg/kg in oil/fat matrix)