AOAC 2018 Methods

Table 2018.03E: Samples considered during validation

Formula category

form

Specificity

Sample name IF powder 1 IF powder 2

Infant Infant

Powder Powder Liquid Liquid Powder Powder Powder Powder Powder

milk Based, for infant – 27.6% fat milk Based, follow up – 26.2% fat calorically dense formula – 10 % fat nutritional supplement – 3.9% fat

Adult Nut 3 liquid Adult Nut 4 liquid

Adult Nutritional Adult Nutritional

SPIFAN Kit II - SRM 1849a- CLC10-B SPIFAN Kit II – 410457651Z SPIFAN Kit II – 4052755861 SPIFAN Kit II – 4044755861

Infant Infant Infant Infant Infant

milk based

partially hydrolyzed soy based

milk-based milk Based

SPIFAN Kit II – 00795RF

elemental powder

Table 2018.03F: Comparison of fat extracted with fat content on a wide range of formula. Different formula category were analyzed (Infant formula, and Adult nutrition), in both powder (p) and liquid (l) form.

Formula category (form) Infant (p) Infant (p) Infant (p) Infant (p) Infant (p) Infant (p) Infant (p) Infant (l) Infant (p) Infant (p) Infant (p) Infant (p) Infant (p) Adult (p) Adult (p) Adult (p) Adult (l) Adult (l) Adult (l) Infant (p) Infant (p) Infant (p) Infant (p) Infant (p) Infant (p) Infant (p) Infant (p) Infant (p)

Fat content (%)

Fat extracted (%)

Difference (%)

Specificity

Standard Infant Formula

27.60 24.80 27.27 25.94 27.70 29.20 26.36 4.00 27.00 18.43 25.60 26.00 26.19 24.60 16.30 20.00 10.00 3.88 15.00 26.9 28.0 25.3 28.0 24.5 18.8 26.5 28.6 27.0 29.0

26.70 23.46 27.85 25.02 29.05 28.31 25.70 3.83 26.91 18.16 25.83 26.55 26.02 24.17 15.71 18.25 9.33 3.64 14.06 25.7 26.4 23.3 28.0 24.7 16.9 25.5 26.3 25.6 28.1

-3.3 -5.4 2.1 -3.5 4.9 -3.0 -2.5 -4.3 -0.3 -1.5 0.9 2.1 -0.6 -1.7 -3.6 -8.8 -6.7 -6.2 -6.3 -4.3 -5.7 -7.9 0.0 0.8 -9.8 -3.7 -8.0 -5.2 -3.1

cream OPO fat Starch

Acidified, starch Partially hydrolyzed

High protein, partially hydrolyzed

Liquid, high protein

Partially hydrolyzed, starch

Milk fortifier

Partially hydrolyzed, soy based Partially hydrolyzed, milk based

Casein

Amino acid based Pea fibers, starch

Starch

High fat, liquid

Yeast

Egg

probiotic LGG

increased protein, vitamins and minerals

rice starch

protein sensitivity

protein allergies; amino acid-based

Toddler

Infant (p) multiple food allergies, amino acid-based

Toddler - lactose sensitivity lactose sensitivity, non-GMO

Low-iron formula