AOAC 2018 Methods

Table 2018.03G : Calibration fit summary of 6 independent calibration curve. Accuracy and reproducibility of the first calibration level are given. Back calc. conc. Accuracy at first level Min-Max (%) (n=6) Reproducibility CV (%) of first calibration level (n=6) Lowest R 2 (n=6) 2-MCPD esters 104-116% 4% 0.9977 3-MCPD esters 91-118% 9% 0.9976 Glycidyl esters 101-119% 7% 0.9983 2-MCPD 104-116% 4% 0.9986 3-MCPD 100-120% 7% 0.9986 Table 2018.03H : LOQ in infant formula and adult nutrition samples, compared with target LOQ. LOQ given in method performance requirements are given for total 3-MCPD (free and bound forms) and total 2-MCPD (free and bound forms) Target LOQ ( μ g/kg) LOQ (sum) ( μ g/kg)

3-MCPD esters

4

25

(6.5)

3-MCPD

2.5

Powder Formula

2-MCPD esters

4

25

(6.5)

2-MCPD

2.5

Glycidyl esters 3-MCPD esters

15

4

0.7 2.5 0.7 2.5 0.7

3

(3.2)

3-MCPD

Liquid Formula

2-MCPD esters

3

(3.2)

2-MCPD

Glycidyl esters

2