AOAC 2018 Methods

AOAC Official Method 2018.07 Fructan (Inulin, FOS, Levan, and Branched Fructan) in Animal Food (Animal Feed, Pet Food, and Ingredients)

Fructan Assay Kit First Action 2018

[This method is based on AOAC Method 999.03, which is commercially available from Megazyme as the Fructan Assay Kit (Megazyme Cat. No. K-FRUC).] See Table 2018.07 for method performance study supporting acceptance of the method. A. Scope of Method (a) (1) Target analyte – Fructan, including inulin, FOS (non-reducing), levan (1), and branched fructans (e.g. agave fructan). (2) Matrices – For this validation, the Fructan Assay Kit (K-FRUC) was tested with the following matrices: (a) Animal feed and pet food samples .—Brett Brothers swine feed (purchased from Glanbia, Ireland). Purina GoCat dry cat food (purchased from Tesco Ireland, Greystones, Ireland). Top Spec TopChop alfalfa (purchased from TopSpec Equine Ltd, Middle Park Farm, Pickhill, Thirsk, North Yorkshire, UK). Timothy grass hay (Lot no. 171201; supplied by Alison Muirhead, Grass Seed Direct, Menstrie Mains, Menstrie, Clackmannanshire, Scotland). Rye grass hay (supplied by Seed Phenomics and Quality Traits Agriculture Victoria Research, Horsham, Victoria, Australia). (b) Barley grain/flour .—Conventional barley flour (Lot no. 60301a; Megazyme, Bray, Ireland); Barley MAX ® (The Healthy Grain Pty. Ltd., South Yarra, Victoria, Australia). (c) Pure, or high purity commercial of purified fructans .—Raftiline ® (Native Chicory Inulin – supplied by BENEO Tienen, Aandorenstraat 1, 3300 Tienen, Belgium); Frutafit ® agave fructan (supplied by Sensus, Borchwerf 3, Roosendaal, The Netherlands); Levan (Cat. No. P-LEVAN; Lot No. 171114 purified from Timothy grass - from Megazyme, Bray, Ireland). (3) Certified reference materials (CRMs).— Certified reference materials are not currently available for fructan. (4) Expression of results – The concentration of fructan is expressed as % w/w of the sample analyzed.

1