AOAC CASP Cannabinoids ERP October Method Book

Quantitating Cannabinoids in Edible Chocolates

Table 1: Method validation summary for phytocannabinoids in a dark chocolate.

Recovery (%)

0.008% w/w

1% w/w

4% w/w

Method Limits (% w/w)

ID Analyte

Calibration Range (% w/w)

Carry- Over (%)

R s

0.008% w/w

1% w/w

4% w/w

RSD r RSD R RSD r RSD R RSD r RSD R

MDL

MRL

1 2 3 4 5 6 7 8 9

CBC

0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5 0.004 - 5

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

1.4 3.8 2.3 2.3 3.1 3.1 2.3 2.3 1.4 4.6 3.3 1.5 1.5 3

105 93.5

101.4 102.9 3.04

6.21 5.32 5.21 7.99 6.15 7.14 6.06 7.84 3.88 6.53 6.53 7.19 3.57 5.47 4.77 7.53 7.1

0.73 1.27 0.96 1.13 1.05 1.79 1.06 0.74 0.63 0.88 1.63 1.46 1.05 1.73 0.94 1.4 1.5

3.68 5.38 3.39 4.28 2.45 3.59 3.03 5.32 1.61 1.97 4.99 3.61 1.99 2.51 1.63 1.97

0.57 0.58 0.54 0.66 0.44 0.44 0.68 0.82 0.86 0.46 0.44 0.64 0.54 0.57 0.51 1.32 0.54

na na na na na na na na na na na na na na na na na

0.00089 0.00051 0.00062 0.00159 0.00053 0.00115 0.00062 0.00134 0.00076 0.00063 0.00049 0.00129 0.00090 0.00066 0.00066 0.00080 0.00152

0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008

CBCA CBD CBDA CBDV

101.5 85.5

2.85

107.8 102.8

101.1 103.1 2.77

99.4 100.4 97.5

92.8 100 94.3

2.51 2.47 1.32 2.85 2.18 3.57 2.29

107 98.3

CBDVA

CBG

101.5 102.3 97.5 105.8 106.3 104.3 101.5

101.2 99.3 100.8 91.9 100.5 97.9 100.5 99.5

CBGA

CBL

10 11 12

CBLA CBN CBNA

100.3 103.3 2.37

98

89

2.63

13 d8-THC 14 d9-THC

100.8 100.7 3.84 101.4 100.2 2.28

102

15 16

THCA THCV

2 4

103.5

99.8 98.1 98.9

99.3

3.11 3.23

101 102

97

17 THCVA

3.3

92.1

2.8

4.2

9