AOAC CASP Cannabinoids ERP October Method Book

AOAC Method Submission Form

Submission Date

2020-08-11 12:39:24

Submitter Name

Alana Greaves

Submitter Email

alana.greaves@canopygrowth.com

Organization

Canopy Growth Corporation

Method Type

Cannabis

Resubmission?

No

Method Name

Method for the Quantification of Cannabinoids in Edible Chocolate by High- Pressure Liquid Chromatography

Method Author(s)

Alana Greaves, Jessica Gauthier, Ben Geiling

Method Applicability

Applicable for determination of 0.005 – 6.25 % w/w cannabinoids in dark, milk, and white chocolate. For some cannabinoids, this range may be extended even further based on instrument linearity. This method accurately quantifies d9-THC, CBD, d9-THCA, CBDA, CBN, and d8-THC, and can precisely monitor an additional 9 cannabinoids. This method has been validated to include chocolate bars, chocolate chips, and chocolate truffles, but does not include chocolates with nuts, fruit, or other ingredients which will not fully homogenize upon melting. This method is suitable for HPLC-DAD as well as HPLC-HDR-DAD. Chocolate samples are melted and extracted with 90:10 methanol:chloroform. After a lipid-removal step, filtered samples are analyzed by gradient reverse- phase high-pressure liquid chromatography with a diode array detector.

A. Principle