AOAC CASP Cannabinoids ERP October Method Book

Table 1. Creation of in-house reference materials.

327

Mass of Input Resin (g)

Mass of Chocolate (g)

In-house Reference Material (No.)

Δ9 -THC

Δ9 -THCA

CBD

CBDA

CBN

Δ8 -THC

Input Resin

N/A

N/A

20.48507 0.10284 0.49994 3.16694 0.10561

3.06546 0.01539 0.07481 0.47391 0.01580

24.64407 0.12372 0.60144 3.80992 0.12705

3.53591 0.01775 0.08629 0.54664 0.01823

1.63578 0.00821 0.03992 0.25289 0.00843

10.86277 0.05453 0.26511 1.67936 0.05600

Cannabis Dark Chocolate (1) Cannabis Milk Chocolate (2) Cannabis White Chocolate (3)

0.09902 0.49918 1.50366 0.10379

19.62526 19.95485 9.72628 20.13191

Cannabis Dark Truffle (4)

328

Table 2. HPLC mobile phase gradient.

329

HPLC Mobile Phase

Pump A Pump B Pump C Pump D

Deionized water

Methanol

Acetonitrile + 0.1% (v/v) phosphoric acid Deionized water + 0.1% (v/v) phosphoric acid

Time (min)

Pump A (%) 1

Pump B (%)

Pump C (%)

Pump D (%)

0.0 7.5

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0

0.0 0.0

66.0 66.0 66.0 66.0 66.0 66.0 66.0 66.0

34.0 34.0 19.0 19.0

10.5 14.0 15.0 21.0 22.0 25.0

15.0 15.0 34.0 34.0

0.0 0.0

0.0 0.0

34.0 34.0

1 Pump A is used for a seal wash.

330

331

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